FastQCFastQC Report
Mon 6 Jun 2016
ERR522905_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522905_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6383310
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT301980.4730774472804861No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT264830.414878801123555No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200880.3146956672948674No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115910.1815829091803469No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90190.14129033369834773No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT78430.12286728985432323No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG107800.054.7942352
ACCTGGG115050.050.2785153
TATAACG6850.050.1182942
ATAACGC8850.039.8549233
GTACCTG161100.037.073941
CCTGGGG99450.036.979344
TAACGCA10000.034.3304944
CTGGGGG62550.027.6669085
CATGGGG151450.025.7420084
ATGGGGG111700.025.6812865
GTACCCG12650.024.9083421
GTACATG266800.024.8891141
GTATAAC19150.024.803511
TGGGGGG140000.024.1407226
TACATGG270250.023.9799082
TACCGGG9250.023.8957212
ACATGGG263950.023.6792143
TACCCGG12000.023.1224982
GAGTACG10250.022.0230731
TATCACG5000.021.6332532
Produced by FastQC (version 0.11.5)