FastQCFastQC Report
Mon 6 Jun 2016
ERR522905_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522905_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6383310
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT310920.4870827204068109No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT271870.42590756206419556No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT248140.38873249145036043No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201890.3162779185093627No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT142980.2239903748995427RNA PCR Primer, Index 17 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111690.1749719189574061No Hit
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCT79400.12438687765438307RNA PCR Primer, Index 17 (95% over 23bp)
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT77650.1216453532728318No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77610.12158268985839635No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG119650.057.3574262
ACCTGGG122650.054.419343
TATAACG8250.045.011412
CCTGGGG102550.043.0390244
GTACCTG172400.040.191251
TAACGCA9350.039.2116784
ATAACGC9950.035.429963
CTGGGGG62450.034.472465
TGGGGGG120950.027.8995116
TATCACG6250.027.8273052
GTATAAC19700.025.0720061
ATGGGGG101750.024.4839065
CATGGGG147450.023.6214144
GTACATG266900.023.4053691
TACATGG270100.022.6239412
ACATGGG256750.022.4080163
GTACCCG13250.022.3661211
TACCCGG11900.021.3302652
TACCGGG8000.021.1525142
GAGTACT229750.019.79172712-13
Produced by FastQC (version 0.11.5)