FastQCFastQC Report
Mon 6 Jun 2016
ERR522904_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522904_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6194767
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143660.2319054130688047No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT121300.19581043161106784No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108730.17551911153397698No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG70900.039.7956622
ACCTGGG82900.033.2979743
GTACCTG102600.028.0961841
TATAACG6250.025.581712
GTACATG236750.025.464331
TACATGG238200.024.7760852
TAACGCA6700.024.5652084
ATAACGC8000.024.1006133
ACATGGG240750.023.341883
CATGGGG155250.022.6264384
GTATCAA311800.022.6229571
ATGGGGG95150.022.1896655
CCTGGGG84750.021.4177954
TCAACGC343550.020.2991734
CAACGCA344300.020.2952775
AACGCAG353250.019.8942836
ATCAACG350750.019.815773
CGCCGTA34950.019.76414594
CGTATCA18800.019.74590794
TATCAAC354250.019.6862052
Produced by FastQC (version 0.11.5)