FastQCFastQC Report
Mon 6 Jun 2016
ERR522904_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522904_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6194767
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140800.2272886131149081No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT127860.20640001472210334No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT123320.19907124836172208No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112130.18100761497567222No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT66050.10662225068352046RNA PCR Primer, Index 17 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG73450.039.1041952
ACCTGGG81900.034.3204273
GTACCTG107200.027.0744041
GTACATG235600.024.5583041
CCTGGGG81350.024.0940784
ATGGGGG90550.024.0860125
TACATGG240500.023.552982
CATGGGG149950.023.4469814
ACATGGG241300.022.8105053
TGGGGGG82600.021.849476
TATAACG5500.021.3673762
GTATCAA325200.019.6145211
GAGTACT145350.019.10929712-13
TCAACGC353200.017.8193444
TAACGCA6100.017.7226964
CAACGCA356950.017.6586195
GTACTTT154100.017.581614-15
ATCAACG360850.017.402643
AGTACTT153700.017.38316312-13
AACGCAG364950.017.308356
Produced by FastQC (version 0.11.5)