FastQCFastQC Report
Mon 6 Jun 2016
ERR522903_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522903_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9342813
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT264660.2832765677746092No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220240.23573200062978894No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118440.1267712411668734No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA398900.026.5023061
GGTATCA289500.025.8772771
TACCTGG86500.025.8245942
TCAACGC449250.023.0925084
GTACATG331600.022.918081
CAACGCA453150.022.8408975
ATCAACG460000.022.5734143
TATCAAC468350.022.3215832
AACGCAG467200.022.1755286
TACATGG334550.021.9430962
ACATGGG335150.021.3425353
ACCTGGG102000.020.9781533
GAGTACT242350.020.3928512-13
CATGGGG202900.020.1878384
ACGCAGA515200.020.0532387
CGCAGAG526400.019.6083988
CGCCGTA42650.019.39091994
GTACCTG121650.019.0583881
GTACTTT258800.018.97903814-15
GCAGAGT567150.017.999379
Produced by FastQC (version 0.11.5)