FastQCFastQC Report
Mon 6 Jun 2016
ERR522903_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522903_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9342813
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT244550.2617520012441649No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT240830.2577703310555397No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218860.2342549294307828No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT128740.13779575808699157TruSeq Adapter, Index 10 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115870.12402046364408663No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT94830.10150047956648603No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA402950.025.4956671
GGTATCA293150.025.4333921
TACCTGG85500.024.6844942
TCAACGC450650.022.3315564
CAACGCA455450.022.0654775
GTACATG316150.021.9464231
ATCAACG459200.021.9259913
AACGCAG469550.021.4401666
TATCAAC473650.021.3762822
TACATGG319900.021.276412
ACATGGG318550.020.3335533
GAGTACT249350.020.29917112-13
ACCTGGG104350.019.6847843
CATGGGG196550.019.3232424
ACGCAGA521300.019.3208967
GTACTTT263550.018.9201414-15
CGCAGAG534500.018.8168668
TATAACG7700.018.3136352
AGAGTAC421100.017.92403210-11
AGTACTT266100.017.45837212-13
Produced by FastQC (version 0.11.5)