FastQCFastQC Report
Mon 6 Jun 2016
ERR522902_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522902_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7096472
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT307830.4337789256407973No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT251040.3537532452745533No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161250.22722558476944602No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139350.1963651797682003No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA73540.10362895816400036No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG138750.059.482272
GTACCTG158650.052.448061
ACCTGGG156900.051.6726533
CCTGGGG133100.040.2012944
TATAACG8800.031.5291842
CTGGGGG81250.029.917245
TAACGCA9950.029.770994
ATAACGC9350.029.1717743
TATCACG7250.028.540272
GTACCCG13650.026.5338171
TGGGGGG132550.025.1363876
GTATCAA392100.024.9641571
CATGGGG182400.024.5407564
GTACACG13500.022.9959751
ATGGGGG120300.022.9417635
GTACATG340950.022.2942261
GGTATCA287750.022.2476671
TACATGG347600.021.754532
TCAACGC448750.021.3328654
ATCAACG452650.021.1940573
Produced by FastQC (version 0.11.5)