FastQCFastQC Report
Mon 6 Jun 2016
ERR522902_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522902_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7096472
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT271150.38209127014099403No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240930.33950672954110156No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156420.22041938585821236No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122540.17267735291564598No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA74180.10453081474851166No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG143800.060.591562
GTACCTG167400.052.4694061
ACCTGGG165200.051.7151263
CCTGGGG135850.041.475664
CTGGGGG84400.032.3494875
TGGGGGG123650.026.0709956
TATAACG8400.025.7359282
ATAACGC9200.025.539713
GTACCCG13100.025.4842471
TAACGCA8950.025.2029844
GGTATCA292300.023.550331
CATGGGG178050.023.5163254
GTACACG15550.022.9809471
GTATCAA385950.022.818731
TATCACG7100.022.5051482
GTACATG338150.022.3038441
ATGGGGG109550.022.263265
TACCGGG11450.022.1640742
TACATGG340800.021.6777522
GTACCGG12350.021.3209151
Produced by FastQC (version 0.11.5)