FastQCFastQC Report
Mon 6 Jun 2016
ERR522901_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522901_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5765135
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102970.17860813320069696No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87320.1514621947274435No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA67830.11765552758088059No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG122200.060.4593962
GTACCTG140950.053.0279241
ACCTGGG138950.052.6296773
CCTGGGG120950.041.4824564
CTGGGGG70250.032.192285
GTACCCG11050.030.6489811
TGGGGGG99850.027.7663526
CATGGGG147100.026.1804524
ATGGGGG90550.024.871395
GTACATG266950.024.386641
TATCACG5800.023.5141242
TACATGG269500.023.4530492
ACATGGG275650.022.076753
TACCCGG14550.021.655622
TATAACG6750.021.5981622
GTATCAA243950.019.9372631
ATAACGC7500.019.4383453
GTACCGG8800.019.2426831
GTACACG12350.019.0435921
TACCGGG8650.019.0287742
Produced by FastQC (version 0.11.5)