##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR522901_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5765135
Sequences flagged as poor quality	0
Sequence length	100
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.18087833155685	33.0	31.0	34.0	27.0	34.0
2	31.517772437245615	34.0	31.0	34.0	27.0	35.0
3	31.487033868244197	33.0	31.0	34.0	27.0	36.0
4	33.781061848508315	35.0	33.0	37.0	30.0	37.0
5	33.8616686339522	35.0	34.0	37.0	30.0	37.0
6	34.08231880086069	35.0	35.0	37.0	30.0	37.0
7	33.95981984810416	35.0	34.0	37.0	30.0	37.0
8	34.04517760642205	35.0	34.0	37.0	30.0	37.0
9	35.20521427512105	37.0	34.0	39.0	30.0	39.0
10-11	35.15321688043731	37.0	34.5	39.0	30.0	39.0
12-13	35.171152019857296	37.0	35.0	39.0	30.0	39.0
14-15	35.927920334909764	38.0	35.0	40.0	30.0	41.0
16-17	36.074421414242686	38.0	36.0	40.0	29.0	41.0
18-19	35.90793433284737	38.0	35.0	40.0	28.5	41.0
20-21	36.00001708546287	38.0	35.0	40.0	28.5	41.0
22-23	35.850839139066125	38.0	35.0	40.0	28.5	41.0
24-25	35.826236159257334	37.5	35.0	40.0	27.5	41.0
26-27	35.716168658669744	37.0	35.5	40.0	27.5	41.0
28-29	35.47252241274489	37.0	34.5	40.0	27.0	41.0
30-31	35.30113293097213	37.0	34.5	40.0	27.0	41.0
32-33	35.27952034080728	37.5	34.0	40.0	26.5	41.0
34-35	35.13565692737464	37.5	34.0	40.0	26.0	41.0
36-37	34.966938154960815	37.5	34.0	40.0	26.0	41.0
38-39	34.810068281141724	36.5	34.0	40.0	25.0	41.0
40-41	34.763514557768374	37.0	33.0	40.0	24.5	41.0
42-43	34.56709521979971	36.0	34.0	40.0	24.0	41.0
44-45	34.49347370009549	37.0	33.0	40.0	24.0	41.0
46-47	34.30301215843167	36.5	33.0	40.0	23.0	41.0
48-49	34.17855748390974	37.0	33.0	40.0	23.0	41.0
50-51	33.170147273220834	36.0	31.5	38.5	21.5	40.0
52-53	33.39814479626236	36.5	32.0	38.5	22.5	39.5
54-55	34.26482432761766	37.0	33.0	39.5	23.0	40.5
56-57	34.41029672332044	37.0	33.0	40.0	24.0	41.0
58-59	34.33518339119553	37.0	33.0	40.0	23.0	41.0
60-61	34.07989448642573	36.5	33.0	39.0	23.0	41.0
62-63	33.89195326735627	36.0	32.5	39.0	22.5	41.0
64-65	33.49400031395622	36.0	33.0	39.0	22.0	41.0
66-67	33.300654364555214	36.0	32.5	38.0	22.0	40.0
68-69	32.956747326818885	35.0	32.0	37.5	21.0	40.0
70-71	32.736487610437564	35.0	31.5	37.0	20.5	39.5
72-73	32.410232110783184	35.0	31.0	37.0	20.0	39.0
74-75	32.03918121605132	35.0	31.0	36.5	20.0	39.0
76-77	31.911097311684806	35.0	31.0	37.0	19.0	38.5
78-79	31.638640292031322	35.0	31.0	36.0	19.0	38.0
80-81	31.372851199494896	35.0	30.0	36.0	18.0	37.5
82-83	31.057397528418676	34.0	30.0	35.5	18.0	37.0
84-85	30.821953085226973	34.0	30.0	35.0	17.5	37.0
86-87	30.57403824888749	34.0	29.0	35.0	17.5	37.0
88-89	30.415508136409642	34.0	29.0	35.0	17.5	36.5
90-91	30.19052997024354	34.0	29.0	35.0	17.0	36.0
92-93	29.930964496061236	34.0	29.0	35.0	16.5	36.0
94-95	29.82925482230685	34.0	28.5	35.0	16.0	36.0
96-97	29.630314467917927	34.0	27.5	35.0	16.5	36.0
98-99	29.53595032900357	34.0	27.0	35.0	15.0	36.0
100	28.241302935664127	32.0	25.0	35.0	10.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.18809395052846156
1101	2	0.18954601293763318
1101	3	0.27961047472936684
1101	4	0.04985005447201729
1101	5	0.09840596930967394
1101	6	0.18734232359295788
1101	7	0.14392794048769986
1101	8	0.16765145963810113
1101	9	0.20129033918890116
1101	10-11	0.19960172703904533
1101	12-13	0.15259976492563965
1101	14-15	0.050847726061292065
1101	16-17	0.10979677814506061
1101	18-19	0.23198213809887136
1101	20-21	0.14096391586111423
1101	22-23	0.12978676221769803
1101	24-25	0.050976397967204434
1101	26-27	0.0174257927924657
1101	28-29	-0.00702319443050925
1101	30-31	-0.02821955224689532
1101	32-33	-0.019596785009134976
1101	34-35	-0.03281679586184083
1101	36-37	-0.07901262256920916
1101	38-39	-0.05650901702315281
1101	40-41	-0.053055466068691715
1101	42-43	-0.01914762391982805
1101	44-45	-0.20126061398897832
1101	46-47	-0.08003231992118032
1101	48-49	-0.009440332571685417
1101	50-51	-0.08986667653901037
1101	52-53	0.020348468309286716
1101	54-55	0.011707155886206522
1101	56-57	0.010460554874889283
1101	58-59	-0.047805320485352354
1101	60-61	0.02500038683190553
1101	62-63	-0.08006489667946681
1101	64-65	-0.170616881809444
1101	66-67	-0.17792606428513835
1101	68-69	-0.15364722010414766
1101	70-71	-0.2503701886806198
1101	72-73	-0.05014724107326174
1101	74-75	-0.11239368371294489
1101	76-77	-0.033865204485138634
1101	78-79	-0.26949422298276815
1101	80-81	-0.19647521320982264
1101	82-83	-0.4132070486269477
1101	84-85	-0.3652401286142357
1101	86-87	-0.3556752317941303
1101	88-89	-0.5236870296766689
1101	90-91	-0.4832403737565869
1101	92-93	-0.3502797994528706
1101	94-95	-0.44668787493476714
1101	96-97	-0.45267106278837943
1101	98-99	-0.3678751303148644
1101	100	-0.4395244703293386
1102	1	-0.1296844098925476
1102	2	-0.08498490343806253
1102	3	-0.10516686833376099
1102	4	-0.03604305387031559
1102	5	-0.04049123358414164
1102	6	-0.030877865309051344
1102	7	8.363029183087178E-4
1102	8	0.026207062642448875
1102	9	0.009211286713330935
1102	10-11	0.019221411030109437
1102	12-13	-0.1080839058523253
1102	14-15	-0.1084841147284763
1102	16-17	-0.057836577377564424
1102	18-19	-0.05196582278937001
1102	20-21	-0.12375009353374367
1102	22-23	-0.039808476924207525
1102	24-25	-0.14690305454353592
1102	26-27	-0.18908185937161903
1102	28-29	-0.15170261679731567
1102	30-31	-0.24127806288519338
1102	32-33	-0.20760102086032362
1102	34-35	-0.2734780784863702
1102	36-37	-0.6758589073289301
1102	38-39	-0.2782070312912879
1102	40-41	-0.9825106615018839
1102	42-43	-0.9527067751167948
1102	44-45	-0.48687878450319033
1102	46-47	-0.46188177607431413
1102	48-49	-0.5489505720903907
1102	50-51	-0.6209255294365903
1102	52-53	-0.47669164824727517
1102	54-55	-0.32463032433183514
1102	56-57	-0.18565087721334095
1102	58-59	-0.21343666327216226
1102	60-61	-0.3738622918337171
1102	62-63	-0.49982621007593053
1102	64-65	-0.25743663362436564
1102	66-67	-0.2553341252763701
1102	68-69	-0.2599849515216661
1102	70-71	-0.3595563180734729
1102	72-73	-0.3064738883138389
1102	74-75	-0.3858823891564356
1102	76-77	-0.49897309466072315
1102	78-79	-0.4366865664226971
1102	80-81	-0.5789861089871593
1102	82-83	-0.629264519927407
1102	84-85	-0.7993056508579492
1102	86-87	-0.7203639328416322
1102	88-89	-0.6859386875932181
1102	90-91	-0.6824231433564272
1102	92-93	-0.6558361120957414
1102	94-95	-0.848756251432544
1102	96-97	-0.7961013486533837
1102	98-99	-0.7588322945239625
1102	100	-0.8901710891843706
1103	1	-0.017949213763788663
1103	2	0.017918284536115436
1103	3	-0.03131930569149333
1103	4	-0.010084104461455468
1103	5	0.005670869322742078
1103	6	0.04320136162720445
1103	7	-0.03089430497040979
1103	8	-0.052560277389737564
1103	9	-0.0072346902190219
1103	10-11	0.0638887483434516
1103	12-13	0.05703464271939396
1103	14-15	-0.010253273808011443
1103	16-17	-0.006842713424667579
1103	18-19	0.03154387839425965
1103	20-21	0.0030473036535667575
1103	22-23	0.036534818027391225
1103	24-25	-0.06221525998600441
1103	26-27	-0.08277671341587478
1103	28-29	-0.16603108555585777
1103	30-31	-0.18925454767233418
1103	32-33	-0.18828086225394713
1103	34-35	-0.26829124364643775
1103	36-37	-0.3606517873842563
1103	38-39	-0.24528809818901465
1103	40-41	-0.1951160627227182
1103	42-43	-0.3179280121043817
1103	44-45	-0.3023586730662231
1103	46-47	-0.2069118127979337
1103	48-49	-0.34708892425905447
1103	50-51	-0.3287067791399707
1103	52-53	-0.12592391438578687
1103	54-55	-0.09686901062797659
1103	56-57	0.08426790031992226
1103	58-59	-0.029519393809096073
1103	60-61	-0.02692918119841181
1103	62-63	-0.1668162200358907
1103	64-65	-0.22279862275834006
1103	66-67	-0.06344593096923035
1103	68-69	-0.19144309646017632
1103	70-71	-0.06348898034184458
1103	72-73	-0.11809162742798662
1103	74-75	-0.12284664737782691
1103	76-77	-0.1859263729598446
1103	78-79	-0.2351248595008748
1103	80-81	-0.0952399171700904
1103	82-83	-0.1376424982413802
1103	84-85	-0.20964257600673264
1103	86-87	-0.9050880536474821
1103	88-89	-0.3552317623913446
1103	90-91	-0.7357982910223022
1103	92-93	-0.2679158927740062
1103	94-95	-0.6407357118188379
1103	96-97	-0.47597634967898017
1103	98-99	-0.4342927554619038
1103	100	-0.4325886122715694
1104	1	0.0024423939984714593
1104	2	-0.053064091685801174
1104	3	-0.02032198802417895
1104	4	-0.05046262983702832
1104	5	-0.08433151690721274
1104	6	0.0679752568955223
1104	7	-0.049229353663129416
1104	8	0.03107479989504469
1104	9	0.01714348173832292
1104	10-11	-0.16751830783542943
1104	12-13	-0.0879310771904187
1104	14-15	-0.1687944579528633
1104	16-17	-0.08078355495748468
1104	18-19	-0.04236644961300584
1104	20-21	-0.18983076065246252
1104	22-23	-0.1493679003204491
1104	24-25	-0.13290199331930097
1104	26-27	-0.08046752271898328
1104	28-29	-0.15072618760947165
1104	30-31	-0.19780827060984052
1104	32-33	-0.22535397466474905
1104	34-35	-0.18131552653342453
1104	36-37	-0.25691217253458376
1104	38-39	-0.17026497902023863
1104	40-41	-0.175576156762844
1104	42-43	-0.3584552950227362
1104	44-45	-0.25183139507470287
1104	46-47	-0.1720518342428008
1104	48-49	-0.24897274852676077
1104	50-51	-0.4391078841960052
1104	52-53	-0.401199599490802
1104	54-55	-0.19901196994398873
1104	56-57	-0.1812593210130018
1104	58-59	-0.15323549780668344
1104	60-61	-0.29245715797922855
1104	62-63	-0.12468988385796109
1104	64-65	-0.1768249875611687
1104	66-67	-0.21489012562319232
1104	68-69	-0.3117730695284706
1104	70-71	-0.3886193648993057
1104	72-73	-0.1826650629569997
1104	74-75	-0.19853439646007942
1104	76-77	-0.2734051690977992
1104	78-79	-0.31333297981021957
1104	80-81	-0.3505265825459247
1104	82-83	-0.12464611765789968
1104	84-85	-0.21658156192961897
1104	86-87	-0.24908248106971698
1104	88-89	-0.14100736842067718
1104	90-91	-0.2832312544653597
1104	92-93	-0.3116751952525476
1104	94-95	-0.2683797999546407
1104	96-97	-0.4220907937933305
1104	98-99	-0.40294523186113906
1104	100	-0.3992069138506338
1105	1	0.11517992203617666
1105	2	0.11199704182564219
1105	3	0.1447455162806115
1105	4	0.14635035126663354
1105	5	0.10987658664247846
1105	6	0.15763516094951058
1105	7	0.06191171571524734
1105	8	0.07412455025939124
1105	9	0.17089857029297661
1105	10-11	0.09375784100818407
1105	12-13	0.2238997253530286
1105	14-15	0.18897148941704955
1105	16-17	0.15593152290168888
1105	18-19	0.1282932283244378
1105	20-21	0.12648281178513798
1105	22-23	0.14685220543099575
1105	24-25	0.19018816092724222
1105	26-27	0.18418900015296913
1105	28-29	0.07772230418444082
1105	30-31	0.10442241451210776
1105	32-33	0.07365830996513978
1105	34-35	0.04111523817061169
1105	36-37	0.01235114870107168
1105	38-39	-0.030988637126043272
1105	40-41	-0.039210739119731386
1105	42-43	0.00571634521353559
1105	44-45	-0.0747355843095221
1105	46-47	0.023483212330496883
1105	48-49	0.016666958682762356
1105	50-51	-0.1686223651025216
1105	52-53	-0.12173354593685559
1105	54-55	-0.016523453532080623
1105	56-57	0.06440297671897355
1105	58-59	0.12567929566027658
1105	60-61	0.08830247428738858
1105	62-63	0.02225875785159559
1105	64-65	0.024736798443818486
1105	66-67	0.0878831957069508
1105	68-69	-0.017060852870272925
1105	70-71	0.03030229845828103
1105	72-73	-0.009144075264003959
1105	74-75	-0.07369834338845038
1105	76-77	0.05159828977683745
1105	78-79	0.14249430095866344
1105	80-81	-0.027761323221696443
1105	82-83	-0.016257355314721167
1105	84-85	-0.06052579343418074
1105	86-87	-0.04237982221755843
1105	88-89	-0.13894623030983055
1105	90-91	-0.1792066875674294
1105	92-93	-0.10582689086561103
1105	94-95	-0.02942234268308752
1105	96-97	-0.08321320762415141
1105	98-99	-0.13216974843912155
1105	100	-0.11772441097041408
1106	1	0.12513584878092843
1106	2	0.1040859082858887
1106	3	0.17153257427370505
1106	4	0.22084518491115546
1106	5	0.1842396155967876
1106	6	0.23338796477266754
1106	7	0.20760201789995136
1106	8	-0.9049758499608132
1106	9	0.16916751108815475
1106	10-11	0.19836543441474674
1106	12-13	0.2061865282324291
1106	14-15	0.18128767648941135
1106	16-17	0.1298128639695051
1106	18-19	0.15574644008675165
1106	20-21	0.1305988865686416
1106	22-23	0.16141158696358104
1106	24-25	0.1821025621552792
1106	26-27	0.24678839982266254
1106	28-29	0.09478345646328279
1106	30-31	0.141124559214731
1106	32-33	0.08723552809486534
1106	34-35	0.20843476890707535
1106	36-37	0.18458352935107314
1106	38-39	0.1837544274713636
1106	40-41	0.1886722578810378
1106	42-43	0.2933395449750691
1106	44-45	0.10705888678714359
1106	46-47	0.20848077406510157
1106	48-49	0.1882137971664264
1106	50-51	0.20659991877419515
1106	52-53	0.32641826952104225
1106	54-55	0.3016567703271491
1106	56-57	0.28316848136829975
1106	58-59	0.3275392103343364
1106	60-61	0.31508785741425527
1106	62-63	0.20770064460540283
1106	64-65	0.1427528793442221
1106	66-67	0.197282362934331
1106	68-69	0.2322860706674703
1106	70-71	0.18531295813225768
1106	72-73	0.17629147005787615
1106	74-75	0.3244684851819528
1106	76-77	0.26240839223009615
1106	78-79	0.114215155616197
1106	80-81	0.15465148299968234
1106	82-83	0.19170228866211758
1106	84-85	0.13560744411622494
1106	86-87	0.11678696338888628
1106	88-89	0.17939750539919075
1106	90-91	0.17246937412784646
1106	92-93	0.10519645361407726
1106	94-95	0.09166223272133323
1106	96-97	0.03545127710446394
1106	98-99	0.10436405400239579
1106	100	0.27668734930913885
1107	1	0.05605901053620599
1107	2	-0.034039449702707
1107	3	0.08221548827950187
1107	4	0.08097423178521979
1107	5	0.13089290310758628
1107	6	0.09097956710824917
1107	7	0.24714319406881202
1107	8	0.19572153319597874
1107	9	0.22110257302552583
1107	10-11	0.11454026749588309
1107	12-13	0.16460615284666602
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2314	96-97	0.43558925193386244
2314	98-99	0.4325751560096194
2314	100	0.4030136964898787
2315	1	-0.05659886960477323
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2315	10-11	-1.3801535468321546
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2315	100	0.3679681277461597
2316	1	-0.20859372342326665
2316	2	-0.4198629504241431
2316	3	-0.2574300075446665
2316	4	-0.20190172496860015
2316	5	-0.26513209475018584
2316	6	-0.1784767894243302
2316	7	-0.13101183229142066
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2316	10-11	-0.2940963155762759
2316	12-13	-0.2304125656852989
2316	14-15	-0.20477285385384647
2316	16-17	-0.40241343538782104
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2316	54-55	-0.058483278479812384
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2316	62-63	-0.11959428596632193
2316	64-65	-0.258753830296925
2316	66-67	-0.22177273581864654
2316	68-69	0.04455126737132531
2316	70-71	0.06928222154888175
2316	72-73	0.0019402937170838186
2316	74-75	0.017046733543068626
2316	76-77	-0.08563962616693743
2316	78-79	-0.14701532532341943
2316	80-81	-0.002705137469529717
2316	82-83	-0.03313287675123533
2316	84-85	0.056702341842488124
2316	86-87	0.09466685425779175
2316	88-89	0.18719989599746611
2316	90-91	0.31725835439963745
2316	92-93	0.32870974063017044
2316	94-95	0.40619025619631444
2316	96-97	0.393543690599401
2316	98-99	0.46228051370575685
2316	100	-0.027665468184885356
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	10345.0
1	965.0
2	565.0
3	490.0
4	389.0
5	348.0
6	419.0
7	696.0
8	1324.0
9	2282.0
10	4452.0
11	8937.0
12	14457.0
13	18552.0
14	21769.0
15	24166.0
16	27933.0
17	32460.0
18	38195.0
19	44154.0
20	50911.0
21	58017.0
22	65575.0
23	71668.0
24	77022.0
25	82667.0
26	91689.0
27	102960.0
28	119579.0
29	141549.0
30	169784.0
31	208535.0
32	263221.0
33	348779.0
34	496334.0
35	770925.0
36	785147.0
37	789764.0
38	683153.0
39	134845.0
40	113.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.47250474918601	17.13181127770127	12.019696441202507	24.375987531910223
2	16.453434349413314	19.547552296668716	39.81804329890429	24.180970055013677
3	19.682135444008644	25.74092502243329	28.60787964028085	25.96905989327722
4	13.21505695713247	15.51331714114849	36.15087093457581	35.12075496714323
5	13.792583250770729	36.16311656169297	33.19985857347828	16.84444161405802
6	31.533441713604788	34.23882684964707	19.336330833908974	14.891400602839175
7	29.150658316238047	31.106477008147998	21.46324148835747	18.279623187256483
8	27.572586278948556	33.49798970706365	20.363304862806356	18.566119151181436
9	28.381035004841277	15.493870008952054	19.561131983388524	36.56396300281815
10-11	26.27874489997043	24.882866795882922	27.784007319298304	21.054380984848347
12-13	26.21685903024826	22.77967860788341	27.643862705122054	23.359599656746287
14-15	23.87033344170464	24.156285912483504	25.458384548917472	26.51499609689438
16-17	23.601585348816123	26.470489791897972	26.301101692064016	23.62682316722189
18-19	23.388749509389378	26.090303308224154	27.525138305677416	22.995808876709052
20-21	24.226276059763123	25.668427576622015	27.101440655978088	23.003855707636777
22-23	23.91402837082542	25.524422782175698	26.930340191341926	23.631208655656955
24-25	23.615453266219813	25.55144560835792	27.042633372471776	23.79046775295049
26-27	23.504289852189544	25.45479805822069	27.15131627222608	23.889595817363684
28-29	23.5102362282774	25.86474567526083	27.153909353094857	23.471108743366916
30-31	24.30356960756929	25.559892800755442	27.062097545331344	23.07444004634393
32-33	23.92041307227504	25.753632141523426	26.979224224391768	23.34673056180977
34-35	23.983600460610035	25.882843145522134	26.934177031638512	23.199379362229315
36-37	24.04303874967212	25.83549114515406	26.856799861102505	23.26467024407132
38-39	23.965081434312246	26.120039677105773	26.6068310593942	23.30804782918778
40-41	23.972742584993554	25.75083978333894	26.902814698099736	23.37360293356777
42-43	23.634371019558234	25.820850233229436	26.722250479283225	23.822528267929105
44-45	23.556790885028008	25.887623809199262	26.769918659042574	23.785666646730157
46-47	23.600468126161775	25.671236130215018	26.642940438752653	24.085355304870554
48-49	23.582154927499428	25.76690530241545	26.812566505264474	23.838373264820646
50-51	23.408432344687572	26.088461280661125	26.799098328496328	23.704008046154982
52-53	23.518894821824972	26.086262050280112	26.449316002319843	23.94552712557507
54-55	23.25572436505706	25.92690051563461	26.87294949674841	23.94442562255992
56-57	23.513887921493968	25.956270966926226	26.472638115081647	24.057202996498162
58-59	23.53343982414234	26.041037511488916	26.643504770782446	23.78201789358629
60-61	23.814945401472446	26.09808226171197	26.710705178383705	23.376267158431883
62-63	23.61420128284654	26.053747233194173	26.7155022261846	23.61654925777469
64-65	23.465247025346276	25.797883024982447	26.677979136336944	24.058890813334337
66-67	23.46421090912759	25.872597852513668	26.54865932981883	24.114531908539906
68-69	23.274347172912485	25.833016277467003	26.68349707910018	24.209139470520327
70-71	23.261423135619793	25.56960957298799	26.895254779383144	24.273712512009073
72-73	23.096833829642275	25.967602358064806	26.606824845278965	24.328738967013955
74-75	23.18371385274337	26.028894844723865	26.46007538027589	24.327315922256872
76-77	22.971197836454586	25.715635991972285	26.788685567539304	24.524480604033826
78-79	22.96218012176743	25.709430909850063	26.769406070038666	24.558982898343842
80-81	23.164488277917826	25.81754577572871	26.47434933117915	24.543616615174315
82-83	23.18607489643615	25.786843923507764	26.635321762126875	24.391759417929208
84-85	23.126516022513563	26.031521526704303	26.28531355622559	24.55664889455654
86-87	23.001930161111144	25.850374509002112	26.56598822005124	24.581707109835506
88-89	22.80901902927402	25.593104161468116	26.7717500372469	24.826126772010962
90-91	23.297934366440014	25.577635023860996	26.72418201565036	24.40024859404863
92-93	23.17086574120368	25.536135228315164	26.748885922847172	24.544113107633983
94-95	23.042616904459308	25.321509060280956	26.797716794066606	24.83815724119313
96-97	22.8906213377748	25.328537611158797	26.850327391789904	24.930513659276503
98-99	23.00478897950353	25.260475544829898	26.66237729283524	25.072358182831326
100	23.078096609861383	25.147284070775676	26.776711138480163	24.997908180882774
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3017.0
1	4096.5
2	3736.0
3	1459.5
4	571.0
5	536.0
6	595.5
7	838.5
8	1260.5
9	1759.0
10	2212.0
11	2583.5
12	2982.5
13	3432.0
14	3870.5
15	4234.5
16	4557.5
17	4955.0
18	5453.5
19	5935.5
20	6477.0
21	7115.0
22	8072.5
23	9526.5
24	11327.5
25	13755.0
26	17485.5
27	22065.0
28	26518.5
29	32230.5
30	39506.5
31	46960.0
32	56058.5
33	67088.0
34	78637.0
35	89354.5
36	102440.0
37	116176.0
38	126619.0
39	137053.0
40	145308.5
41	153065.0
42	162028.0
43	171643.0
44	181947.5
45	192731.0
46	205335.0
47	219249.5
48	238632.0
49	254727.0
50	265525.0
51	262880.5
52	246095.5
53	237394.5
54	232138.0
55	220096.5
56	203265.0
57	187152.5
58	171678.0
59	152214.0
60	127144.0
61	102591.5
62	83517.5
63	65801.5
64	49359.5
65	37202.5
66	28034.5
67	21826.0
68	17263.5
69	13319.5
70	10494.5
71	8279.0
72	6603.0
73	5246.0
74	4151.5
75	3196.5
76	2252.5
77	1492.5
78	979.0
79	634.0
80	420.5
81	296.5
82	207.5
83	144.5
84	122.5
85	94.0
86	65.5
87	44.0
88	41.0
89	39.5
90	30.0
91	26.5
92	22.5
93	15.0
94	7.0
95	4.5
96	5.5
97	3.0
98	2.0
99	3.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1449922681775882
2	0.07292110245466932
3	0.31388683872970885
4	0.3107993134592685
5	0.3118920892572334
6	0.2603755159246054
7	0.2603755159246054
8	0.2598031095542429
9	0.27086963271458514
10-11	0.24822488979009166
12-13	0.2579211067910812
14-15	0.23078384114162115
16-17	0.24015916366225595
18-19	0.2540790458506176
20-21	0.2549636738775415
22-23	0.24494656239619716
24-25	0.24600464689898846
26-27	0.24493788957240378
28-29	0.2550070379965083
30-31	0.23917046174981157
32-33	0.2526827212198847
34-35	0.23645586790248624
36-37	0.24512001887206455
38-39	0.24650767067900406
40-41	0.2626131044632953
42-43	0.24908349934563545
44-45	0.2349121052672661
46-47	0.23753129805286433
48-49	0.24596995560381502
50-51	0.2396561398822404
52-53	0.2558483019044654
54-55	0.26585674056201636
56-57	0.2628993076484766
58-59	0.2607050832287535
60-61	0.24077493415158535
62-63	0.26884886477072956
64-65	0.26257841316812186
66-67	0.26774741614897135
68-69	0.270982379423899
70-71	0.292751167145262
72-73	0.3013112442293199
74-75	0.28014955417349285
76-77	0.27844968070999204
78-79	0.27573508686266673
80-81	0.26174582208395814
82-83	0.27167620532736875
84-85	0.2833151348580736
86-87	0.27534480979196496
88-89	0.28333248050566034
90-91	0.2775823983306549
92-93	0.25775632313900715
94-95	0.27949909238899
96-97	0.2772441582027134
98-99	0.26481600170681174
100	0.2870704675606035
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5765135.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.948372144328694
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.31895615543361	42.487141565287864
2	16.23703120240697	18.81819053272312
3	5.143132842729208	8.941085278745739
4	2.0073089236227273	4.652811380588866
5	0.966856435320489	2.8013878262045386
6	0.5303575252525372	1.8440013145727538
7	0.34961261998731935	1.4181637546565267
8	0.238366155656566	1.1050344555679734
9	0.1764021397149251	0.9199995155330709
>10	0.9393873755393103	9.674169384918033
>50	0.06003487459301509	2.380410158326728
>100	0.03066749045378796	3.1658178712815683
>500	0.0011548528781759994	0.4618275896908326
>1k	6.159211884917167E-4	0.7541641470191761
>5k	7.699014856136095E-5	0.3460972770052906
>10k+	3.849507428068047E-5	0.229697947877762
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10297	0.17860813320069696	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8732	0.1514621947274435	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	6783	0.11765552758088059	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.7345647586743416E-5	0.0
5	0.0	0.0	0.0	1.7345647586743416E-5	0.0
6	6.938259034697367E-5	0.0	0.0	1.7345647586743416E-5	3.469129517348683E-5
7	6.938259034697367E-5	0.0	0.0	1.7345647586743416E-5	3.469129517348683E-5
8	8.672823793371708E-5	0.0	0.0	1.7345647586743416E-5	3.469129517348683E-5
9	8.672823793371708E-5	0.0	0.0	1.7345647586743416E-5	3.469129517348683E-5
10-11	9.540106172708879E-5	0.0	0.0	3.469129517348683E-5	1.2141953310720391E-4
12-13	1.040738855204605E-4	8.672823793371708E-6	0.0	3.469129517348683E-5	1.2141953310720391E-4
14-15	1.040738855204605E-4	1.7345647586743416E-5	0.0	3.469129517348683E-5	1.9947494724754927E-4
16-17	1.040738855204605E-4	1.7345647586743416E-5	0.0	3.469129517348683E-5	2.341662424210361E-4
18-19	1.040738855204605E-4	1.7345647586743416E-5	0.0	3.469129517348683E-5	2.7753036138789466E-4
20-21	1.040738855204605E-4	1.7345647586743416E-5	0.0	6.070976655360196E-5	3.122216565613815E-4
22-23	1.040738855204605E-4	1.7345647586743416E-5	0.0	7.805541414034537E-5	3.5558577552824E-4
24-25	1.127467093138322E-4	1.7345647586743416E-5	0.0	1.6478365207406244E-4	4.423140134619571E-4
26-27	1.300923569005756E-4	1.7345647586743416E-5	0.0	4.16295542081842E-4	4.683324848420722E-4
28-29	1.3876518069394733E-4	1.7345647586743416E-5	0.0	0.0017085462872942262	4.683324848420722E-4
30-31	1.3876518069394733E-4	1.7345647586743416E-5	0.0	0.006513290668822153	4.7700530863544393E-4
32-33	1.3876518069394733E-4	1.7345647586743416E-5	0.0	0.02339927859451687	5.897520179492761E-4
34-35	1.3876518069394733E-4	5.203694276023025E-5	0.0	0.05401434658511899	6.070976655360195E-4
36-37	1.3876518069394733E-4	6.938259034697367E-5	0.0	0.10813276705575844	6.070976655360195E-4
38-39	1.3876518069394733E-4	9.540106172708879E-5	0.0	0.20119216635863688	6.070976655360195E-4
40-41	1.4743800448731905E-4	1.040738855204605E-4	0.0	0.31582955125942413	6.24443313122763E-4
42-43	1.5611082828069075E-4	1.3876518069394733E-4	0.0	0.4274661391277047	6.331161369161347E-4
44-45	1.6478365207406244E-4	1.3876518069394733E-4	0.0	0.5674628608003108	6.417889607095063E-4
46-47	1.7345647586743416E-4	1.3876518069394733E-4	0.0	0.7361492835813905	6.851530796763649E-4
48-49	1.7345647586743416E-4	1.3876518069394733E-4	0.0	0.9147660896058809	8.32591084163684E-4
50-51	1.7345647586743416E-4	1.3876518069394733E-4	0.0	1.1029750387458404	8.32591084163684E-4
52-53	1.7345647586743416E-4	1.3876518069394733E-4	0.0	1.2968560146466648	8.759552031305424E-4
54-55	1.9080212345417758E-4	1.6478365207406244E-4	0.0	1.5235636286054013	9.19319322097401E-4
56-57	2.341662424210361E-4	1.7345647586743416E-4	0.0	1.7775906375132586	9.279921458907726E-4
58-59	2.4283906621440783E-4	1.7345647586743416E-4	0.0	2.0653722766249185	9.366649696841444E-4
60-61	2.4283906621440783E-4	1.7345647586743416E-4	0.0	2.359467731458153	9.540106172708879E-4
62-63	2.601847138011512E-4	1.7345647586743416E-4	0.0	2.6674223587131958	9.80029088651003E-4
64-65	2.601847138011512E-4	1.7345647586743416E-4	0.0	2.9788287698380005	0.0010060475600311182
66-67	2.601847138011512E-4	1.7345647586743416E-4	0.0	3.319497288441641	0.0010320660314112333
68-69	2.601847138011512E-4	1.7345647586743416E-4	0.0	3.716677926882892	0.0010494116789979766
70-71	2.601847138011512E-4	1.7345647586743416E-4	0.0	4.1472402641048305	0.0010580845027913484
72-73	2.601847138011512E-4	1.7345647586743416E-4	0.0	4.556337362438174	0.001214195331072039
74-75	2.601847138011512E-4	1.7345647586743416E-4	0.0	4.963691223189049	0.001214195331072039
76-77	2.601847138011512E-4	1.7345647586743416E-4	0.0	5.401937682291915	0.001214195331072039
78-79	2.601847138011512E-4	1.7345647586743416E-4	0.0	5.892498614516399	0.001214195331072039
80-81	2.601847138011512E-4	1.7345647586743416E-4	0.0	6.406155276502632	0.0012488866262455258
82-83	2.7753036138789466E-4	1.7345647586743416E-4	0.0	6.927296585422544	0.0012835779214190126
84-85	3.122216565613815E-4	1.7345647586743416E-4	0.0	7.459643182683493	0.0012835779214190126
86-87	3.122216565613815E-4	1.7345647586743416E-4	0.0	8.003273123699618	0.001309596392799128
88	3.122216565613815E-4	1.7345647586743416E-4	0.0	8.438726933541018	0.0013182692165924995
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCTGG	12220	0.0	60.459396	2
GTACCTG	14095	0.0	53.027924	1
ACCTGGG	13895	0.0	52.629677	3
CCTGGGG	12095	0.0	41.482456	4
CTGGGGG	7025	0.0	32.19228	5
GTACCCG	1105	0.0	30.648981	1
TGGGGGG	9985	0.0	27.766352	6
CATGGGG	14710	0.0	26.180452	4
ATGGGGG	9055	0.0	24.87139	5
GTACATG	26695	0.0	24.38664	1
TATCACG	580	0.0	23.514124	2
TACATGG	26950	0.0	23.453049	2
ACATGGG	27565	0.0	22.07675	3
TACCCGG	1455	0.0	21.65562	2
TATAACG	675	0.0	21.598162	2
GTATCAA	24395	0.0	19.937263	1
ATAACGC	750	0.0	19.438345	3
GTACCGG	880	0.0	19.242683	1
GTACACG	1235	0.0	19.043592	1
TACCGGG	865	0.0	19.028774	2
>>END_MODULE