FastQCFastQC Report
Mon 6 Jun 2016
ERR522901_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522901_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5765135
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89040.15444564611236336No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78860.13678777686905857No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA68670.11911256197816704No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG125000.062.5250322
ACCTGGG142550.054.032283
GTACCTG149400.052.8734441
CCTGGGG124250.044.7056734
CTGGGGG71400.035.6071175
TATAACG6850.032.246232
GTACCCG12650.029.7357161
TGGGGGG88550.029.3469546
TAACGCA8050.028.6049864
ATAACGC8250.025.6330413
CATGGGG145400.023.6585564
TACCCGG15450.023.4225392
ATGGGGG88500.022.51445
TATCACG5850.021.6912
GTACATG274000.021.2961041
GTACACG14150.020.6022261
TACATGG280500.020.3403072
ACATGGG279300.019.938343
TACACGG13600.019.0062072
CTGGGGA69250.018.5259575
Produced by FastQC (version 0.11.5)