FastQCFastQC Report
Mon 6 Jun 2016
ERR522900_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522900_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8817080
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225660.25593507147491007No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186270.2112604172810046No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108730.12331747018287233No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA126700.051.2849461
GTATCAA227250.041.8234981
TCAACGC277200.033.7219664
CAACGCA280950.033.2876435
ATCAACG281300.033.250953
TATCAAC289200.032.526232
AACGCAG293600.031.8285436
ACGCAGA335900.027.7505867
CGCAGAG344800.026.937378
GTACATG284500.026.510881
TACATGG288300.025.5999982
ACATGGG292050.024.5594183
GCAGAGT386500.023.5190689
CATGGGG189300.022.170334
GAGTACT226700.020.69282312-13
CAGAGTA373800.020.49086610-11
AGAGTAC345950.020.02212710-11
GTACTTT243550.019.0688314-15
GAGTACA216850.017.5751251
AGTACTT246550.017.44522312-13
Produced by FastQC (version 0.11.5)