FastQCFastQC Report
Mon 6 Jun 2016
ERR522900_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522900_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8817080
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206180.23384158927899032No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181550.20590717108158255No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104740.11879216248463209No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA128350.052.0810241
GTATCAA229700.039.5869641
TCAACGC280750.031.8205384
CAACGCA287950.030.9922435
ATCAACG288550.030.9456673
TATCAAC299700.030.0821062
AACGCAG300050.029.8045756
ACGCAGA345200.025.8786547
CGCAGAG355550.024.9857568
GTACATG285900.024.3516771
TACATGG293350.023.072
ACATGGG298400.022.0178893
GCAGAGT409400.021.4808969
GAGTACT225850.020.36459712-13
AGAGTAC356850.019.6656910-11
CAGAGTA391850.019.26463310-11
CATGGGG200300.019.1214944
GTACTTT242500.018.80927514-15
GTGGTAT75650.017.7843671
AGTACTT247100.017.6907112-13
Produced by FastQC (version 0.11.5)