FastQCFastQC Report
Mon 6 Jun 2016
ERR522899_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522899_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3933975
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124680.3169313480639811No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104640.26599050578613237No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53000.13472378446736444No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG49750.040.4586262
GGTATCA89050.038.4994051
ACCTGGG55350.034.835873
GTACCTG63100.033.0913121
GTATCAA137850.032.8874861
TCAACGC161100.027.7901384
ATCAACG161300.027.6397673
CAACGCA163300.027.4438485
TATCAAC166600.026.8169252
AACGCAG169900.026.533026
TAACGCA4500.025.0811864
ACGCAGA192400.023.2570517
GTACATG127550.023.080981
CGCAGAG196950.022.813488
TACATGG128700.022.3266242
CCTGGGG54750.022.2466684
TATCACG4450.021.136372
ACATGGG130450.020.8734823
GCAGAGT212150.020.6694189
GAGTACT120100.020.56481712-13
Produced by FastQC (version 0.11.5)