FastQCFastQC Report
Mon 6 Jun 2016
ERR522899_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522899_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3933975
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116070.29504508798352813No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101930.2591017990709143No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52650.13383409909824032No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG49000.041.0632062
GGTATCA88600.038.550361
ACCTGGG55800.034.877373
GTATCAA138050.033.7383881
GTACCTG62550.032.5676271
TCAACGC161200.028.2285044
CAACGCA163900.027.7925195
ATCAACG163950.027.697673
TATAACG4450.027.4674592
ATAACGC4650.027.2953323
TATCAAC166600.027.2870522
AACGCAG171400.026.7906956
TAACGCA4750.026.7206924
CCTGGGG50000.024.256454
ACGCAGA192900.023.8043927
GTACATG132050.023.157971
CGCAGAG197750.023.1483828
TACATGG132400.022.5116042
TATACCG2756.002665E-1122.2225425
ACATGGG131750.021.4794643
Produced by FastQC (version 0.11.5)