FastQCFastQC Report
Mon 6 Jun 2016
ERR522898_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522898_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5374472
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161360.30023414393078984No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127930.23803268488513848No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75720.14088825841868746No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA85500.047.9913251
TACCTGG54300.040.7721142
GTATCAA148600.039.5508651
GTACCTG68950.033.3041151
TCAACGC179700.032.1925134
ACCTGGG68550.031.811773
ATCAACG181650.031.7199443
CAACGCA184400.031.3951325
TATCAAC187700.030.8273322
AACGCAG192250.030.3011076
GTACATG169300.028.4335441
ACGCAGA216800.026.8046157
TACATGG175650.026.7337272
CGCAGAG224500.025.8850178
ACATGGG181150.025.0101363
CATGGGG127550.024.8695684
CCTGGGG64750.023.2975464
GCAGAGT247300.023.1363059
TATAACG5900.023.1040522
ATGGGGG82050.022.9083585
Produced by FastQC (version 0.11.5)