FastQCFastQC Report
Mon 6 Jun 2016
ERR522898_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522898_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5374472
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145280.2703149258196898No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123590.22995747303176944No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68590.12762183894529547No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA84950.047.570031
GTATCAA145800.039.1710171
TACCTGG57550.037.8352
TCAACGC175750.031.9253464
CAACGCA179300.031.3456675
ATCAACG179900.031.1375063
GTACCTG72100.030.9928861
TATAACG4750.030.6920662
TATCAAC184250.030.577882
AACGCAG187050.030.2225076
ACCTGGG71100.030.0732613
GTACATG171500.026.3336331
ACGCAGA218550.025.780247
TACATGG174500.025.1715222
CGCAGAG224700.025.122548
TATCACG6450.024.7900432
ACATGGG177950.023.9259383
CATGGGG127700.023.367354
TAACGCA6650.022.6128314
ATAACGC7100.022.5039793
Produced by FastQC (version 0.11.5)