FastQCFastQC Report
Mon 6 Jun 2016
ERR522897_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522897_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6621054
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230250.3477543001461701No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189500.28620820793789026No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103020.1555945624367359No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG87450.046.251652
ACCTGGG99200.038.924533
GTACCTG130800.031.0295331
GTATCAA279750.029.066791
GGTATCA195550.028.1865541
TATCACG5550.027.9646632
CCTGGGG89350.027.8451614
TCAACGC324300.024.813714
CAACGCA326950.024.653885
GTACATG237150.024.6501871
ATCAACG327700.024.4416283
TATCAAC334250.024.1172642
AACGCAG337850.024.0420786
TACATGG241600.023.6520042
TATAACG5550.022.8801782
ATGGGGG91250.022.7280125
ACATGGG241550.022.5277713
CATGGGG146450.022.3515934
ACGCAGA377200.021.4333117
CGCAGAG385300.020.9336138
Produced by FastQC (version 0.11.5)