FastQCFastQC Report
Mon 6 Jun 2016
ERR522897_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522897_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6621054
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT219860.3320619345499976No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT203480.3073226709825958No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191220.28880598164582255No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT108400.16372015694177997RNA PCR Primer, Index 46 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100030.15107866511887683No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG90750.046.8244362
ACCTGGG100750.040.728683
TATAACG6800.033.8717772
GTACCTG128900.033.2806131
ATAACGC7600.030.3049533
CCTGGGG88650.029.3739364
TAACGCA7350.028.7777124
GGTATCA215650.028.6176381
GTATCAA302850.026.559381
TCAACGC341500.023.2058754
CAACGCA344200.023.0243645
ATCAACG346900.022.8446453
GTACATG233650.022.7489871
AACGCAG354950.022.4829046
TATCAAC355000.022.4435832
TACATGG236100.021.920082
ACATGGG236400.020.9173
CATGGGG139950.020.8233174
ATGGGGG86050.020.812065
GAGTACT204650.020.7256212-13
Produced by FastQC (version 0.11.5)