FastQCFastQC Report
Mon 6 Jun 2016
ERR522896_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522896_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13095300
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT394560.30129893931410506No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT311750.2380625109772208No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175970.1343764556749368No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA550300.027.4247761
TACCTGG132950.027.2740022
GGTATCA393600.026.3706071
GTACATG519150.024.531761
GTACCTG154450.024.329421
TACATGG528400.023.702342
TCAACGC632400.023.5374684
CAACGCA638800.023.2342345
ATCAACG646350.023.036743
TATCAAC659450.022.7077452
AACGCAG657700.022.6786696
ACATGGG531600.022.6481553
CATGGGG305800.021.9772134
ACCTGGG165300.021.196363
TATAACG12300.020.6476922
ACGCAGA732800.020.2884677
GAGTACT349800.020.1268612-13
CGCAGAG752200.019.7396838
GTACTTT371700.018.67656114-15
GTATAGG36750.018.4279921
Produced by FastQC (version 0.11.5)