FastQCFastQC Report
Mon 6 Jun 2016
ERR522896_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522896_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13095300
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT368020.2810321260299497No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT317900.24275885241269768No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176040.13442990996769835No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA394050.027.620391
GTATCAA551500.027.3849871
TACCTGG128950.027.2301432
GTACATG515000.023.6634431
GTACCTG153400.023.639961
TCAACGC633250.023.5370354
CAACGCA640150.023.2248655
ATCAACG651750.022.9121193
TACATGG526150.022.7099722
TATCAAC661950.022.6382982
AACGCAG658900.022.6329676
ACATGGG527900.021.7342783
ACCTGGG160250.020.8545423
CATGGGG310400.020.7003444
GAGTACT359900.020.45614212-13
ACGCAGA736800.020.2272837
CGCAGAG759950.019.6167688
GTACTTT380400.019.00770414-15
AGTACTT379200.018.0888512-13
AGAGTAC633200.017.88031810-11
Produced by FastQC (version 0.11.5)