FastQCFastQC Report
Mon 6 Jun 2016
ERR522895_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522895_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8837200
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT272260.30808400850948264No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220490.24950210473905762No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128580.14549857420902548No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA183850.038.924211
GTATCAA295450.035.4005241
TCAACGC348350.029.409974
CAACGCA353600.028.986275
ATCAACG356150.028.742083
TATCAAC364500.028.177432
AACGCAG366500.028.0494126
GTACATG298200.026.4278241
TACATGG300800.025.5497952
ACGCAGA413750.024.8348317
ACATGGG311700.023.8993323
CGCAGAG430150.023.8004538
CATGGGG223900.022.2487494
GCAGAGT466800.021.5582969
GAGTACT250350.021.08493212-13
ATGGGGG131700.020.2323445
AGAGTAC380850.019.92173210-11
GTACTTT272950.019.14955514-15
CAGAGTA455050.018.7123110-11
AGTACTT267900.017.89727812-13
Produced by FastQC (version 0.11.5)