FastQCFastQC Report
Mon 6 Jun 2016
ERR522895_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522895_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8837200
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT248080.2807224007604219No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT213860.24199972842076675No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123420.13965962069433757No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA182350.037.2287861
GTATCAA284450.034.2359731
TCAACGC342400.028.1491874
CAACGCA347250.027.7424965
ATCAACG347450.027.7273143
TATCAAC357450.027.090022
AACGCAG359500.026.9145096
GTACATG295900.025.8519821
TACATGG301450.024.8056182
ACGCAGA409250.023.6310637
ACATGGG307350.023.5927723
CGCAGAG425850.022.6927138
CATGGGG220100.021.1799814
GAGTACT250750.020.80681612-13
GCAGAGT467950.020.500349
AGAGTAC378800.019.75401910-11
ATGGGGG128550.019.4113795
GTACTTT270500.019.0253414-15
AGTACTT266300.018.60343212-13
GAGTACA234100.018.0264131
Produced by FastQC (version 0.11.5)