FastQCFastQC Report
Mon 6 Jun 2016
ERR522894_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522894_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6495065
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180480.27787250781939826No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143910.2215682214111791No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88360.13604174861991375No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87700.048.2821731
GTATCAA158250.041.1765481
TACCTGG67800.038.4073832
TCAACGC190950.033.8143584
ATCAACG194650.033.100963
CAACGCA195050.032.957745
TATCAAC200900.032.3342442
AACGCAG203150.031.6835526
GTACCTG84250.031.1607671
ACCTGGG82150.030.7774223
GTACATG198950.028.9928151
TACATGG201100.027.9780032
ACGCAGA231200.027.7786317
CGCAGAG239350.026.8118848
ACATGGG209300.026.2034913
CATGGGG151150.024.1883094
ATGGGGG99500.023.1414385
GCAGAGT274650.023.1076419
TATCACG6550.020.810922
GTGGTAT50200.020.8062151
Produced by FastQC (version 0.11.5)