FastQCFastQC Report
Mon 6 Jun 2016
ERR522894_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522894_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6495065
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164950.25396204656920296No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139750.21516335864229227No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87820.13521034816433708No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA90300.047.8737141
GTATCAA156500.039.9165531
TACCTGG65700.037.508372
TCAACGC192550.032.0197644
ATCAACG194300.031.8044173
CAACGCA195450.031.5927545
TATCAAC204050.030.491972
GTACCTG81300.030.3186651
AACGCAG206250.030.1432676
ACCTGGG79850.029.4258863
GTACATG197200.027.1936361
TACATGG198050.026.5478382
ACGCAGA237000.026.2717297
CGCAGAG245400.025.341768
ACATGGG204450.025.0078453
CATGGGG147100.024.1191544
TATAACG5150.023.7426032
ATGGGGG94650.023.036965
GCAGAGT277950.022.3571419
TGGGGGG89700.020.4313286
Produced by FastQC (version 0.11.5)