FastQCFastQC Report
Mon 6 Jun 2016
ERR522893_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522893_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7477923
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT288000.3851336794989732No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT234910.3141380300385548No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135660.18141401028066217No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG95000.041.6300962
GTACCTG112700.035.800071
ACCTGGG109050.035.490263
GGTATCA202550.033.3614581
GTATCAA300250.030.6655081
GTACATG263650.025.9867671
TCAACGC352100.025.803184
ATCAACG355350.025.6202933
CAACGCA357600.025.4298935
TATCAAC361900.025.2215612
TATAACG6550.025.1282232
CCTGGGG108300.025.0107174
AACGCAG369350.024.8048866
TACATGG271150.024.748532
CATGGGG187350.023.7197594
ACATGGG275250.023.5427363
ATGGGGG118000.022.5936645
ACGCAGA411150.022.2251637
TAACGCA7650.022.1295814
CGCAGAG422800.021.6118958
Produced by FastQC (version 0.11.5)