FastQCFastQC Report
Mon 6 Jun 2016
ERR522893_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522893_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7477923
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT274640.3672677560333264No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT235670.31515435502612155No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136310.18228323559897583No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG94350.043.092272
ACCTGGG107800.036.4059643
GTACCTG115600.035.9292071
GGTATCA221650.031.9384251
TATAACG7650.029.492052
GTATCAA320300.029.0184881
TAACGCA10150.026.3945144
ATAACGC9350.025.1340943
CCTGGGG109700.024.7642974
TCAACGC370350.024.7218764
ATCAACG375650.024.4106163
CAACGCA376150.024.3031945
TATCAAC381900.024.1230162
GTACATG264750.023.8252541
AACGCAG387900.023.7105186
TACATGG273700.022.5483672
CATGGGG179850.021.8735794
ACATGGG273750.021.6847323
ACGCAGA430900.021.3663757
ATGGGGG111600.020.8471095
Produced by FastQC (version 0.11.5)