FastQCFastQC Report
Mon 6 Jun 2016
ERR522892_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522892_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7231315
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT293760.4062331678263221No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT239770.3315717818958239No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151020.208841683704831No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122250.1690563887757621No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG121200.054.15962
GTACCTG140350.047.2177961
ACCTGGG142750.045.326273
CCTGGGG124800.034.3579834
GGTATCA212350.029.9012031
TATAACG8650.029.8977992
GTATCAA304600.028.7512631
TAACGCA9850.028.161984
ATAACGC9500.026.2337273
TATCACG6850.025.3982492
CATGGGG184150.024.9953044
GTACATG312050.024.4173221
CTGGGGG80350.024.1652095
ATGGGGG114750.024.1317585
TCAACGC362750.023.8353614
TACATGG315400.023.6447162
TATCAAC368200.023.5616252
ATCAACG367800.023.4730033
CAACGCA368800.023.4687085
TGGGGGG126750.023.0241286
Produced by FastQC (version 0.11.5)