FastQCFastQC Report
Mon 6 Jun 2016
ERR522892_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522892_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7231315
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT254710.35223192462228514No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT228920.316567595243742No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149240.20638016736928208No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109370.1512449672016777No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG123850.055.742742
GTACCTG146850.047.9314581
ACCTGGG143050.047.566173
CCTGGGG126000.037.257394
GGTATCA224950.029.9733961
TATAACG8150.029.4086512
GTATCAA316250.027.25241
CTGGGGG74450.026.825125
ATAACGC9100.025.8193953
TAACGCA9800.023.0161484
TCAACGC370350.022.9912874
CAACGCA374000.022.7794745
ATCAACG373950.022.7322563
TATCAAC377400.022.6388572
AACGCAG383750.022.3969466
TGGGGGG111050.022.385186
CATGGGG167200.022.2871424
CTATAGC24400.021.9549543
GTACATG297900.021.3234581
ATGGGGG104050.020.9101125
Produced by FastQC (version 0.11.5)