FastQCFastQC Report
Mon 6 Jun 2016
ERR522891_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522891_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8403416
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT229690.27332932226608797No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192070.22856181343396542No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117210.13947899282863063No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA118500.052.6139341
GTATCAA208850.041.6586951
TCAACGC254250.033.5674934
CAACGCA258000.033.0605935
ATCAACG262300.032.57453
AACGCAG268150.031.8375876
TATCAAC271050.031.5973822
GTACATG278450.028.2041051
TACATGG278400.027.6902432
ACGCAGA309900.027.3817867
CGCAGAG326250.026.0223988
ACATGGG288850.025.7733
CATGGGG197050.023.1344574
GCAGAGT369750.022.4764799
GAGTACT218550.020.4973612-13
CAGAGTA353350.020.06950410-11
AGAGTAC327050.019.83037410-11
GAGTACA209750.019.113531
GTGGTAT60750.018.8993451
GTACTTT236700.018.76768714-15
Produced by FastQC (version 0.11.5)