FastQCFastQC Report
Mon 6 Jun 2016
ERR522891_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522891_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8403416
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT208770.24843468418081407No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187600.22324254803046759No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112540.13392172897307475No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA120500.051.8456571
GTATCAA211250.040.8417171
TCAACGC262700.032.3254364
CAACGCA267000.031.7696365
ATCAACG267700.031.7229963
TATCAAC277150.030.7670612
AACGCAG277600.030.6575586
GTACATG286200.027.7462431
TACATGG290200.026.7579422
ACGCAGA317250.026.7519047
ACATGGG296250.025.4296023
CGCAGAG333650.025.4007478
CATGGGG191100.024.2718454
GCAGAGT375300.022.3811689
AGAGTAC340300.020.00079310-11
ATGGGGG101050.019.951125
CAGAGTA365150.019.65016210-11
GAGTACT223500.019.64315612-13
GAGTACA222050.019.067471
GTGGTAT66500.018.8174741
Produced by FastQC (version 0.11.5)