FastQCFastQC Report
Mon 6 Jun 2016
ERR522890_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522890_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6432534
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170340.2648101043849904No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138080.21465879542960828No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78930.12270436502939588No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA94750.050.5999221
GTATCAA168100.040.4160961
TCAACGC199450.033.7625664
CAACGCA205350.032.792775
ATCAACG205100.032.7663043
TATCAAC214250.031.4564062
AACGCAG216350.031.332916
TACCTGG58700.030.744962
GTACATG213300.027.7488291
ACGCAGA245450.027.5226677
TACATGG216200.026.9119852
CGCAGAG252900.026.6743328
ACATGGG219800.025.9779993
GTACCTG74600.025.1645361
CATGGGG152800.023.8650134
ACCTGGG73650.023.737083
GCAGAGT284400.023.2069329
ATGGGGG92700.022.8117925
GAGTACT163000.021.14125812-13
CAGAGTA276600.020.32885710-11
Produced by FastQC (version 0.11.5)