FastQCFastQC Report
Mon 6 Jun 2016
ERR522890_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522890_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6432534
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157490.24483352905713363No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133180.2070412686508925No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77160.1199527278052475No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA90400.048.7613831
GTATCAA158200.039.431371
TCAACGC195050.031.5135364
ATCAACG197050.031.1469523
CAACGCA200700.030.6029685
TACCTGG59050.030.3443682
TATCAAC207600.029.6541422
AACGCAG209550.029.4002066
GTACATG208250.025.7302251
ACGCAGA242750.025.3399417
TACATGG213450.024.5890142
CGCAGAG252450.024.2973128
GTACCTG76600.024.074771
ACATGGG211950.023.8354783
ACCTGGG74050.023.3545533
CATGGGG142300.021.5977334
ATGGGGG86900.021.4687145
GCAGAGT285450.021.2412179
AGAGTAC254800.019.36940810-11
GAGTACT165050.019.35086612-13
Produced by FastQC (version 0.11.5)