FastQCFastQC Report
Mon 6 Jun 2016
ERR522889_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522889_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5603957
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT336760.6009325196463856No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220410.3933113690915187No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175890.3138675046935585No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT99930.17832042608463983No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99110.1768571743145067No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC77660.1385806493518776No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC69230.12353770737355764No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA57860.10324847246329692No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG206600.026.7356341
TACCTGG42750.025.5091442
TACATGG216700.025.0968062
ACATGGG217450.024.0381493
CATGGGG147100.023.5178414
GTACCTG52050.021.8563441
ATGGGGG90700.021.8176085
GAGTACT181250.021.61880312-13
TATAACG4650.020.2172032
AGAGTAC280850.019.89714610-11
GTACTTT201600.019.31984714-15
TATCACG4950.018.9919152
ACCTGGG57600.018.688253
AGTACTT190950.018.6383112-13
TAACGCA4650.018.1949924
GTATAGG14100.016.6699311
ACTTTTT246150.015.94655416-17
GTATAGC20200.015.8248811
GAGTACA161850.015.7133041
TGGGGGG92000.015.6264276
Produced by FastQC (version 0.11.5)