FastQCFastQC Report
Mon 6 Jun 2016
ERR522889_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522889_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5603957
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT344230.6142623863816229No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT203740.36356453127673893No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170430.30412438924852564No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT99190.17699993058476357No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93870.16750663861268028No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC81530.14548648392555474No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC72640.12962269339325766No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG57240.10214211136880601No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG42950.029.1118222
GTACATG200150.025.7084731
TATAACG3600.024.8085882
TACATGG207700.024.5099772
GTACCTG53250.024.201681
ACATGGG211450.023.447623
CATGGGG142100.022.0589984
ACCTGGG57700.021.0949253
GAGTACT182650.020.80857312-13
ATGGGGG82500.020.7346945
AGAGTAC276200.019.13042310-11
GTACTTT202100.018.67603514-15
AGTACTT190800.017.92406712-13
GTATACG3652.6175258E-916.7519381
ATAACGC6500.015.9060873
TGGGGGG82150.015.9033046
GTATAGG17950.015.7217671
ACTTTTT246300.015.44641916-17
GAGTACA158750.015.3768781
TATCACG4652.6011548E-1015.1631432
Produced by FastQC (version 0.11.5)