FastQCFastQC Report
Mon 6 Jun 2016
ERR522888_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522888_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11151919
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT852000.7639940713342699No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT336310.3015714156460426No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT269330.24151000379396587No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT244210.21898473258279583No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC176280.15807144940704823No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161870.14514990648694634No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG142970.1282021506791791No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA139810.1253685576446529No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA114630.10278948403409315No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG435000.030.0899181
TACATGG447000.028.9432132
ACATGGG453250.027.8196873
CATGGGG303600.025.9050454
ATGGGGG199150.023.7946995
GAGTACT295400.020.02366312-13
AGAGTAC485950.019.06893210-11
GAGTACA324300.019.0497571
CATGGGA153750.018.8957254
TGGGGGG192900.018.5353266
GTACTTT317000.018.4828414-15
AGTACAT312350.018.4524822
AGTACTT317550.017.69486812-13
GTATAGG33200.016.1438771
TACCTGG71900.015.4962022
CATGGGT67600.015.3686534
ACTTTTT391500.015.28329316-17
TACTTTT376050.014.84961114-15
GTGTAGC53350.014.717131
ATGGGGA109700.014.6989895
Produced by FastQC (version 0.11.5)