FastQCFastQC Report
Mon 6 Jun 2016
ERR522888_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522888_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11151919
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT868980.7792201503615656No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT313740.2813327464089364No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT259770.23293748815786772No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT238600.2139542082398554No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC190290.1706343096645519No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC180560.16190935389684952No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154130.13820939696567022No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG143620.12878501000590123No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG453350.029.8230921
TACATGG462850.028.6666872
ACATGGG468300.027.4765153
CATGGGG313850.025.9046844
ATGGGGG203750.024.3105815
GAGTACT305700.019.91626212-13
AGAGTAC501950.018.84052810-11
CATGGGA158800.018.6442264
TGGGGGG180000.018.5891636
GTACTTT324000.018.50483514-15
GAGTACA337300.018.4037361
AGTACAT325250.017.8032912
GTATAGG32350.017.5897451
AGTACTT328900.017.18201412-13
CATGGGT71250.015.5661584
ACTTTTT393100.015.33265716-17
TACCTGG73150.014.9709192
TACTTTT384850.014.38199514-15
GTATAGA35650.014.378561
ATGGGGA111850.014.1174315
Produced by FastQC (version 0.11.5)