FastQCFastQC Report
Mon 6 Jun 2016
ERR522887_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522887_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4500033
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171330.38073054130936373No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136100.3024422265347832No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76060.1690209827350155No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG62200.048.6137242
ACCTGGG69700.041.628033
GTACCTG81400.037.203621
GGTATCA114450.035.7048951
TATAACG5150.034.6988142
GTATCAA176250.033.2706381
ATAACGC5900.032.678743
CCTGGGG66500.029.9117974
TAACGCA5950.029.2420984
TCAACGC203850.028.443054
ATCAACG205950.028.0851523
CAACGCA206650.028.0570375
TATCAAC211200.027.4095842
AACGCAG215200.027.1299236
GTACATG166050.027.0167621
TACATGG171600.025.5135362
TATCACG4250.025.4493792
ACATGGG169550.024.712313
GTATAAC12000.024.2959691
CATGGGG109700.024.0909254
Produced by FastQC (version 0.11.5)