FastQCFastQC Report
Mon 6 Jun 2016
ERR522887_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522887_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4500033
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159250.35388629372273495No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137450.30544220453494453No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75950.16877654008315052No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG68900.049.802722
ACCTGGG77200.043.532063
TATAACG4900.043.168392
TAACGCA5650.039.9312824
GTACCTG90550.038.4909971
GGTATCA114450.036.2889441
ATAACGC6500.032.540153
GTATCAA174750.032.4877431
CCTGGGG67400.032.288034
TCAACGC207400.027.240574
CAACGCA209800.026.9295485
ATCAACG209200.026.9163133
TATCAAC212350.026.651622
AACGCAG219500.026.0155646
TATCACG5350.025.479662
GTACATG164950.024.6086581
CATGGGG103250.024.0361164
CTGGGGG43550.023.9604475
TACATGG168150.023.957052
GGGTTAG19500.023.1561361
Produced by FastQC (version 0.11.5)