FastQCFastQC Report
Mon 6 Jun 2016
ERR522886_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522886_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8374050
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT655400.7826559430621981No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260170.310685988261355No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206120.24614135334754392No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT199080.23773442957708635No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC151150.18049808635009346No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC138710.1656426699147963No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA112770.13466602181740017No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112160.13393758097933497No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG111950.1336868062645912No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA96340.11504588580197156No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG277250.025.9108921
TACATGG280800.024.9629272
ACATGGG288950.023.8051243
GAGTACT230550.021.42788512-13
CATGGGG189500.021.111534
GTACTTT251350.019.40278214-15
AGAGTAC363600.019.03730410-11
AGTACTT244750.018.02511412-13
GAGTACA220300.016.1551861
ACTTTTT312050.015.846251516-17
GTGTAGC46450.015.4858941
CATGGGA109600.015.3557784
ATGGGGG102600.015.3502335
AGTACAT213500.015.2598522
TACTTTT297100.014.81775914-15
GTATATA42600.014.457471
TAGCCCT48250.014.41995054
TATACTG38350.014.0977575
ATGGGGA85750.013.980535
ATACCGT8900.013.7249886
Produced by FastQC (version 0.11.5)