FastQCFastQC Report
Mon 6 Jun 2016
ERR522886_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522886_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8374050
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT662930.7916480078337245No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT234440.27996011487870265No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197580.23594318161463093No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT195810.23382950901893348No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC157750.1883795773848974No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC145580.17384658558284222No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106850.1275965631922427No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC87630.10464470596664698No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG285050.024.6478751
TACATGG289050.024.0496642
ACATGGG295350.022.801093
GAGTACT234600.021.22221612-13
CATGGGG191950.020.4186464
GTACTTT254050.019.4644214-15
AGAGTAC367700.019.28098310-11
AGTACTT247100.018.39824912-13
GTATAGG25200.017.5419241
GAGTACA223900.017.0760081
ATGGGGG104400.016.3850335
ACTTTTT310650.016.12697816-17
AGTACAT222900.016.0678862
CATGGGA110800.015.1842124
TACTTTT301650.015.02947714-15
GTATAAT39750.014.4335311
GTGTAGC46600.014.431121
GTATATG34400.014.0808431
GTATATA42100.014.0746751
GTATAGA31750.014.0711561
Produced by FastQC (version 0.11.5)