FastQCFastQC Report
Mon 6 Jun 2016
ERR522885_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522885_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5632422
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT252370.44806656887569857No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT203740.3617271575176718No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133960.23783729273126197No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112320.19941687607924266No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA61780.10968638358418456No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG117600.056.2211272
GTACCTG132650.050.320361
ACCTGGG133450.048.944653
CCTGGGG115650.037.3659444
TATAACG6200.035.647392
TATCACG5800.028.376682
ATAACGC7800.028.3351043
CTGGGGG71700.027.0179985
TAACGCA8950.026.7949664
GTATCAA315850.025.5716041
GTACATG267850.024.5518441
CATGGGG150000.024.294984
TGGGGGG108050.024.0070086
TACATGG276500.023.3845562
GGTATCA232550.023.0598831
ATGGGGG93900.022.9337485
GTATAAC14650.022.797611
ACATGGG279650.022.3812733
TCAACGC361200.021.9881154
GTACACG12450.021.914281
Produced by FastQC (version 0.11.5)