FastQCFastQC Report
Mon 6 Jun 2016
ERR522885_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522885_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5632422
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT229990.4083323302124734No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207690.3687401263612705No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129170.22933295836142958No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98130.1742234512967956No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA59440.10553186533253367No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG118150.059.1072122
ACCTGGG133200.051.4720843
GTACCTG137250.051.1103131
TATAACG7850.040.7093932
CCTGGGG111100.040.139584
ATAACGC8900.034.847743
TAACGCA8950.034.6530574
CTGGGGG64800.029.2247435
GTACCCG11750.027.60981
GGTATCA244750.024.3199731
TGGGGGG97400.024.2197286
GTATAAC16200.024.0887971
GTATCAA317850.022.6614971
TATCACG5000.022.557792
ATGGGGG85750.022.1943055
TACCCGG14300.021.6901862
CATGGGG142000.021.6095394
GTACATG274450.021.1742131
TACATGG279600.020.4890522
GTACACG12000.019.9820941
Produced by FastQC (version 0.11.5)