##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR522885_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5632422 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.240124230748336 33.0 31.0 34.0 30.0 34.0 2 32.36088684406104 34.0 31.0 34.0 30.0 34.0 3 32.55485047107621 34.0 31.0 34.0 30.0 35.0 4 35.652108631064934 37.0 35.0 37.0 33.0 37.0 5 35.58740342964359 36.0 35.0 37.0 33.0 37.0 6 35.71032053351116 37.0 35.0 37.0 33.0 37.0 7 35.55644800762443 37.0 35.0 37.0 33.0 37.0 8 35.54472818265393 36.0 35.0 37.0 33.0 37.0 9 36.84137250369379 38.0 37.0 39.0 33.0 39.0 10-11 36.642554215575466 37.5 35.0 39.0 33.0 39.0 12-13 36.64379409071266 37.5 35.0 39.0 33.0 39.0 14-15 37.817324412126794 38.0 37.0 41.0 33.0 41.0 16-17 37.63090940274006 38.0 36.5 41.0 33.0 41.0 18-19 37.54515881444963 38.0 36.0 41.0 32.5 41.0 20-21 37.56309878769737 38.0 36.5 41.0 33.0 41.0 22-23 37.41803890049432 38.0 36.0 41.0 33.0 41.0 24-25 37.34180739653385 38.0 36.5 41.0 32.0 41.0 26-27 37.02784423823357 38.0 36.0 40.0 32.5 41.0 28-29 36.98892554570662 38.0 36.0 40.0 32.0 41.0 30-31 36.73988197262208 38.0 35.5 40.0 32.0 41.0 32-33 36.67229515118008 37.5 36.0 40.0 30.5 41.0 34-35 36.43647457523602 38.0 35.0 40.0 30.5 41.0 36-37 36.210374506739726 37.0 35.5 40.0 30.5 41.0 38-39 36.157218244655674 37.0 35.0 40.0 30.0 41.0 40-41 36.33938179703155 37.5 35.0 40.0 30.5 41.0 42-43 36.374975188293774 37.5 35.5 40.0 30.5 41.0 44-45 36.18568353010481 37.0 35.0 40.0 30.5 41.0 46-47 36.06167914975121 37.0 35.5 40.0 29.5 41.0 48-49 35.83555049674901 38.0 35.0 40.0 29.5 41.0 50-51 35.66515222048348 38.0 34.5 40.0 28.5 41.0 52-53 35.67544894540927 37.5 35.0 40.0 28.5 41.0 54-55 35.480517972552484 37.5 34.0 40.0 28.0 41.0 56-57 35.2379830559571 37.0 35.0 40.0 27.5 41.0 58-59 35.05587445329913 37.0 34.0 40.0 27.0 41.0 60-61 34.87000894109141 37.0 34.0 39.0 26.5 41.0 62-63 34.61638838496121 36.5 34.0 39.0 26.0 41.0 64-65 34.433905964432356 37.0 33.5 39.0 25.5 40.5 66-67 33.93635828423368 35.5 33.5 38.0 26.0 40.0 68-69 33.79990215576886 36.5 33.0 37.5 25.0 40.0 70-71 33.38244772852602 35.0 33.0 37.0 25.0 39.5 72-73 33.15196126994746 35.0 33.0 37.0 24.5 39.0 74-75 32.96266668229049 35.0 32.5 37.0 23.5 39.0 76-77 31.35838063980291 34.0 30.0 36.0 22.0 37.0 78-79 32.46749755256265 35.0 32.0 36.5 23.0 37.0 80-81 32.575535533381554 35.0 32.0 36.0 24.0 37.5 82-83 32.46979913791971 35.0 32.0 37.0 23.5 37.0 84-85 32.133493726144806 35.0 32.0 36.0 23.0 36.5 86-87 32.00841902471086 35.0 32.0 36.0 23.0 36.5 88-89 31.959313329150408 35.0 32.0 36.0 22.5 37.0 90-91 31.718951367635448 34.5 32.0 35.0 23.0 36.5 92-93 31.4946167918526 35.0 31.0 35.0 21.0 36.5 94-95 31.623132375379544 35.0 31.0 35.0 21.0 37.0 96-97 31.34399206948627 35.0 31.0 35.0 20.5 37.0 98-99 31.188432791435016 34.0 31.0 35.0 20.0 36.0 100 29.979014356523713 34.0 29.0 35.0 18.0 36.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.060209623354943176 1101 2 -0.1282930592686995 1101 3 -0.127578231925483 1101 4 -0.09683175017761414 1101 5 -0.22569728667308198 1101 6 -0.11788956984998578 1101 7 -0.04067244901172273 1101 8 -0.19896571312222022 1101 9 -0.1254352061391799 1101 10-11 -0.19833987692742028 1101 12-13 -0.15020574483752114 1101 14-15 -0.2838598809443127 1101 16-17 -0.31299364951212993 1101 18-19 -0.39775233556751033 1101 20-21 -0.29251270808762797 1101 22-23 -0.7422385591803646 1101 24-25 -0.284643970177882 1101 26-27 -0.20988455924437233 1101 28-29 -0.3302715747254439 1101 30-31 -0.27314764804280145 1101 32-33 -0.2530296534583911 1101 34-35 -0.39580405618793435 1101 36-37 -0.4148762560372674 1101 38-39 -0.36593178426382167 1101 40-41 -0.3649498994328084 1101 42-43 -0.3012805989024443 1101 44-45 -0.40039867073056 1101 46-47 -0.2841926753295212 1101 48-49 -0.4521865007852526 1101 50-51 -0.5495689095115353 1101 52-53 -0.5561235767533645 1101 54-55 -0.48685848157906975 1101 56-57 -0.547187162235808 1101 58-59 -0.6253670121279526 1101 60-61 -0.7323446321904186 1101 62-63 -0.6365140220584422 1101 64-65 -0.6129469383115307 1101 66-67 -0.5155464120101101 1101 68-69 -0.4890193593743106 1101 70-71 -0.6392088518357966 1101 72-73 -0.7133173058789097 1101 74-75 -0.5734971356569503 1101 76-77 -0.6794026008011969 1101 78-79 -0.585539530987532 1101 80-81 -0.5252367576988632 1101 82-83 -0.4617996596712288 1101 84-85 -0.5256929595126607 1101 86-87 -0.6542359108398585 1101 88-89 -0.5888003316248138 1101 90-91 -0.5911981263732109 1101 92-93 -0.5560853105907633 1101 94-95 -0.6851859653401533 1101 96-97 -0.7395116578219039 1101 98-99 -0.7616051599440574 1101 100 -0.6577347522186123 1102 1 -0.0556926058935332 1102 2 -0.11362284459602989 1102 3 -0.1427122519536752 1102 4 -0.11538744027124892 1102 5 -0.09872478034699128 1102 6 -0.13782600748896812 1102 7 -0.06636393437547383 1102 8 -0.1440782708389179 1102 9 -0.15937710633633628 1102 10-11 -0.20776825136690746 1102 12-13 -0.1291480284131481 1102 14-15 -0.2499725794854939 1102 16-17 -0.2425851779904704 1102 18-19 -0.264051313371759 1102 20-21 -0.291965808717805 1102 22-23 -0.18192792901078292 1102 24-25 -0.12413452007064052 1102 26-27 -0.10344093762969919 1102 28-29 -0.13375280267085543 1102 30-31 -0.2737934377212383 1102 32-33 -0.141118345899649 1102 34-35 -0.1979024545056376 1102 36-37 -0.28118320081782144 1102 38-39 -0.34926865541644503 1102 40-41 -0.23751405234809653 1102 42-43 -0.2827241172733679 1102 44-45 -0.15101252367455942 1102 46-47 -0.19798300543697422 1102 48-49 -0.206195369698996 1102 50-51 -0.023852920963605584 1102 52-53 -0.19364583954808978 1102 54-55 -0.13073367667534797 1102 56-57 -0.23285503299995725 1102 58-59 -0.1796012655971495 1102 60-61 -0.22709783625707303 1102 62-63 -0.35265263478176934 1102 64-65 -0.3891025903839349 1102 66-67 -0.3724543551990678 1102 68-69 -0.21869121493437405 1102 70-71 -0.25129059762434025 1102 72-73 -0.3130451278128916 1102 74-75 -0.43272114596921085 1102 76-77 -0.28588425053175825 1102 78-79 -0.333797309853729 1102 80-81 -0.3023266624971086 1102 82-83 -0.21086002106290636 1102 84-85 -0.33793347354541226 1102 86-87 -0.2679271579170255 1102 88-89 -0.34599063959679555 1102 90-91 -0.3959664941940879 1102 92-93 -0.33464332435714894 1102 94-95 -0.4578044692087637 1102 96-97 -0.5308900335140834 1102 98-99 -0.6462776542388049 1102 100 -0.5986966357260677 1103 1 -0.071551176743192 1103 2 -0.13022868006813582 1103 3 -0.1966187763356828 1103 4 -0.05505419699149883 1103 5 -0.17144354301077414 1103 6 -0.2049274528489562 1103 7 -0.17192748969088711 1103 8 -0.204922928410177 1103 9 -0.19095200840306603 1103 10-11 -0.27469618598801304 1103 12-13 -0.1900253078944445 1103 14-15 -0.22523429770783565 1103 16-17 -0.32414272222928986 1103 18-19 -0.3629575000528291 1103 20-21 -0.3232019967496882 1103 22-23 -0.4415166092755385 1103 24-25 -0.29771860760271807 1103 26-27 -0.3075072620927557 1103 28-29 -0.2930530863396399 1103 30-31 -0.5088242360462374 1103 32-33 -0.2908148203019252 1103 34-35 -0.40607299347632875 1103 36-37 -0.40835529321280006 1103 38-39 -0.33437334270538344 1103 40-41 -0.4133947088387089 1103 42-43 -0.5475887667397998 1103 44-45 -0.45835792418786525 1103 46-47 -0.5061821139065117 1103 48-49 -0.460268650801261 1103 50-51 -0.5865738449133602 1103 52-53 -0.3615816763709958 1103 54-55 -1.19631614324296 1103 56-57 -0.4734110913546772 1103 58-59 -0.47638500192025646 1103 60-61 -0.4331365367838842 1103 62-63 -0.44705807852079005 1103 64-65 -0.39142009680025325 1103 66-67 -0.37915461185177435 1103 68-69 -0.369613948961117 1103 70-71 -0.5180598128073726 1103 72-73 -0.6168800595973067 1103 74-75 -0.6288832424165989 1103 76-77 -0.49466677113151647 1103 78-79 -0.3598423591580584 1103 80-81 -1.267715626430654 1103 82-83 -0.43152103686731635 1103 84-85 -0.3797055254162771 1103 86-87 -0.4196455828798804 1103 88-89 -0.43806047614954835 1103 90-91 -0.36708752620813456 1103 92-93 -0.38490483995893143 1103 94-95 -0.3977924470555827 1103 96-97 -0.5404492662575642 1103 98-99 -0.40305409622178345 1103 100 -0.5756607043468982 1104 1 -0.081639897650291 1104 2 -0.1981276257613942 1104 3 -0.13941832543719812 1104 4 -0.11351477652866748 1104 5 -0.13614919913437262 1104 6 -0.28788919278662917 1104 7 -0.30848514501878554 1104 8 -0.1904334383233106 1104 9 -0.30595114639125853 1104 10-11 -0.22740588693621788 1104 12-13 -0.2622884867287354 1104 14-15 -0.335556889047929 1104 16-17 -0.32152744472779915 1104 18-19 -0.3625327934683895 1104 20-21 -0.3424856649414494 1104 22-23 -0.33975976291256416 1104 24-25 -0.27389817129213156 1104 26-27 -0.35264312841461276 1104 28-29 -0.40781835389483945 1104 30-31 -0.5339624231691076 1104 32-33 -0.38295519560859503 1104 34-35 -0.42677798651392607 1104 36-37 -0.5592879236749653 1104 38-39 -0.3707379736980201 1104 40-41 -0.2701141571511485 1104 42-43 -0.3320204357272658 1104 44-45 -0.39898149026439 1104 46-47 -0.3380668695593201 1104 48-49 -0.3770408144508792 1104 50-51 -0.28812407367802706 1104 52-53 -0.3689563199622654 1104 54-55 -0.35368375812260666 1104 56-57 -0.38276736746368556 1104 58-59 -0.3962142572746856 1104 60-61 -0.37169604875258244 1104 62-63 -0.3654940575672754 1104 64-65 -0.45074056626514647 1104 66-67 -0.4048041708995811 1104 68-69 -0.29243160031050053 1104 70-71 -0.3849098227536629 1104 72-73 -0.4082015899997984 1104 74-75 -0.4194209388558292 1104 76-77 -0.40792955211421145 1104 78-79 -0.35407695898566516 1104 80-81 -0.4056773663683231 1104 82-83 -0.3199132522376473 1104 84-85 -0.9504467229495965 1104 86-87 -0.44551137427302123 1104 88-89 -0.4035411471925805 1104 90-91 -0.2560210849907065 1104 92-93 -0.2934008282886147 1104 94-95 -0.33120952815100324 1104 96-97 -0.24783710526028457 1104 98-99 -0.2933874958637155 1104 100 -1.6640878643495505 1105 1 -0.017307332550466015 1105 2 -0.12499319421922905 1105 3 -0.15643966896807626 1105 4 -0.0329993998184932 1105 5 -0.020022043672341283 1105 6 -0.07133084782104504 1105 7 -0.13114593613617131 1105 8 -0.16351904936411898 1105 9 -0.13568702631380347 1105 10-11 -0.13809874189855265 1105 12-13 -0.10427328285167903 1105 14-15 -0.21183109636533715 1105 16-17 -0.19298426868425622 1105 18-19 -0.24167486539958816 1105 20-21 -0.22485136583995313 1105 22-23 -0.2008531052254483 1105 24-25 -0.15950187155211637 1105 26-27 -0.20617647821563878 1105 28-29 -0.21676464279880037 1105 30-31 -0.1632444122491563 1105 32-33 -0.15031841858507278 1105 34-35 -0.18390414847681313 1105 36-37 -0.2023876293537583 1105 38-39 -0.17915760707430906 1105 40-41 -0.13571203545954802 1105 42-43 -0.17580230790222373 1105 44-45 -0.23486139338462664 1105 46-47 -0.24169968579149526 1105 48-49 -0.291488420382052 1105 50-51 -0.19937785950011744 1105 52-53 -0.3856065577728742 1105 54-55 -1.212051677499261 1105 56-57 -0.34193350397932676 1105 58-59 -0.2682175445994446 1105 60-61 -0.2266437172143867 1105 62-63 -0.13616451025022513 1105 64-65 -0.1706621660551022 1105 66-67 -0.23512137210607875 1105 68-69 -0.11503141434982922 1105 70-71 -0.068768335736344 1105 72-73 -0.11497893977286111 1105 74-75 -0.19650427704402063 1105 76-77 -0.15484787933818467 1105 78-79 -0.08429145071259114 1105 80-81 -0.09637851732038172 1105 82-83 -0.010268931145567706 1105 84-85 -0.04618811488928287 1105 86-87 -0.05873823463706884 1105 88-89 -0.2558712677718411 1105 90-91 -0.23393213846488692 1105 92-93 -0.07680082859932824 1105 94-95 -0.18204716677675847 1105 96-97 -0.16367213391174218 1105 98-99 -0.11062375446556061 1105 100 -0.04740337062630573 1106 1 -0.010789435339944475 1106 2 -0.13216872744214925 1106 3 -0.12619731100855347 1106 4 -0.09875525065481838 1106 5 -0.09302959218172902 1106 6 -0.11717428777504324 1106 7 -0.05887333629711122 1106 8 -0.1319997886568487 1106 9 -0.1852063578272265 1106 10-11 -0.16134937556572737 1106 12-13 -0.14021137340125733 1106 14-15 -0.1592966190343077 1106 16-17 -0.17026515954041344 1106 18-19 -0.20985654440470825 1106 20-21 -0.28137409509159284 1106 22-23 -0.11925409191867686 1106 24-25 -0.11839507578862651 1106 26-27 -0.12846266518022986 1106 28-29 -0.10351212415717015 1106 30-31 -0.10384604442653256 1106 32-33 -0.03703159723568206 1106 34-35 -0.11008802247249605 1106 36-37 -0.14464296780236197 1106 38-39 -0.14526691454234708 1106 40-41 -0.18283300010718762 1106 42-43 -0.1449886687246078 1106 44-45 -0.13629282576427926 1106 46-47 -0.23679037985439777 1106 48-49 -0.2362894790296295 1106 50-51 -0.18852696097967936 1106 52-53 -0.15080825482398552 1106 54-55 -0.3117276436921941 1106 56-57 -0.22078150710245126 1106 58-59 -0.2272740813942491 1106 60-61 -0.20729507635751787 1106 62-63 -0.19752765297149466 1106 64-65 -0.15706054440296668 1106 66-67 -0.11705283941037692 1106 68-69 -0.0671871744777377 1106 70-71 -0.05608929904440885 1106 72-73 -0.05427367227393631 1106 74-75 -0.06564686244436757 1106 76-77 -0.07041193163139425 1106 78-79 0.0010613409154487385 1106 80-81 0.07218039578611979 1106 82-83 0.05872816675924497 1106 84-85 0.07007443047688611 1106 86-87 0.1892169508128383 1106 88-89 0.011133593931315744 1106 90-91 -0.03262260747906609 1106 92-93 -0.009405289195232314 1106 94-95 0.028300912415129886 1106 96-97 0.13007486998354878 1106 98-99 -0.09019197092569797 1106 100 -0.00991783086817577 1107 1 -0.023447739544351975 1107 2 -0.13525020271509902 1107 3 -0.1517199648997689 1107 4 -0.04269056062786092 1107 5 -0.09698096671780831 1107 6 -0.1666550468097725 1107 7 -0.16877875856335578 1107 8 -0.14230872988785137 1107 9 -0.1761900424538183 1107 10-11 -0.13633929552864998 1107 12-13 -0.19304426067310487 1107 14-15 -0.27574827136668745 1107 16-17 -0.12757413886918556 1107 18-19 -0.2796166712703112 1107 20-21 -0.20685634946410403 1107 22-23 -0.16163064718109155 1107 24-25 -0.11323322372864197 1107 26-27 -0.13255127108729425 1107 28-29 -0.11509773780124988 1107 30-31 -0.1156125431455024 1107 32-33 -0.06590701432697443 1107 34-35 -0.166062071536075 1107 36-37 -0.25585609629663963 1107 38-39 -0.2024411229816394 1107 40-41 -0.19484443579020194 1107 42-43 -0.2327949986704141 1107 44-45 -0.28012535110723746 1107 46-47 -0.2193340573694016 1107 48-49 -0.20730302278969504 1107 50-51 -0.1834794022535462 1107 52-53 -0.18014608369512075 1107 54-55 -0.30319953546910483 1107 56-57 -0.2022806705387481 1107 58-59 -0.25987681046507305 1107 60-61 -0.2833832441401327 1107 62-63 -0.347582974119895 1107 64-65 -0.3611843113842568 1107 66-67 -0.3169422764839567 1107 68-69 -0.298267659208463 1107 70-71 -0.22326218993554647 1107 72-73 -0.30476473675760474 1107 74-75 -0.298214112959478 1107 76-77 -0.18974964938400518 1107 78-79 -0.13337143079314018 1107 80-81 -0.017482089649412558 1107 82-83 -0.021904589710231903 1107 84-85 -0.06222367511110605 1107 86-87 -0.12433109524737063 1107 88-89 -0.14018768463011 1107 90-91 -0.13782668457626457 1107 92-93 -0.043311200382959214 1107 94-95 0.016711568363625418 1107 96-97 0.072916297507291 1107 98-99 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0.05166855757090616 2316 82-83 -0.022433971111887274 2316 84-85 -0.676094764076943 2316 86-87 -0.04946759307892634 2316 88-89 -0.017843499673151797 2316 90-91 0.1304073069731615 2316 92-93 0.24925752227169795 2316 94-95 0.2618145905851783 2316 96-97 0.21526027029898387 2316 98-99 0.30995033034358954 2316 100 0.0883475805085645 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 4.0 11 5.0 12 28.0 13 183.0 14 763.0 15 2111.0 16 4591.0 17 8704.0 18 14620.0 19 20935.0 20 27587.0 21 35278.0 22 45336.0 23 51274.0 24 56147.0 25 61868.0 26 70270.0 27 81561.0 28 98544.0 29 118939.0 30 146455.0 31 180161.0 32 225589.0 33 291212.0 34 414352.0 35 733739.0 36 1143287.0 37 836247.0 38 800071.0 39 162476.0 40 85.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.034034261618515 17.78172494611375 12.55994095825603 23.6242998340117 2 16.783582088227153 20.328084080379284 39.23029541452549 23.658038416868067 3 19.327759887309583 26.477597026643245 29.50462518610999 24.690017899937185 4 13.175366473114062 16.324198987821365 36.3333957696579 34.16703876940667 5 13.490461085696653 36.20201469994507 33.42873963896753 16.87878457539075 6 30.473769627661003 34.891621205162046 19.46250111630505 15.172108050871907 7 28.071836249456496 31.877464415390822 21.903121233302695 18.147578101849987 8 27.187394971858453 33.63031052909949 20.538013951021757 18.64428054802031 9 27.922676958509147 15.721602536173604 20.258549519194407 36.09717098612285 10-11 25.663517902557544 25.298878999023778 28.04348893277962 20.99411416563906 12-13 25.882800456821048 23.35573496389862 28.069540484722104 22.691924094558228 14-15 23.480075053593854 24.588974678911853 26.432935366511302 25.498014900982984 16-17 22.75534403849918 26.977532822798388 27.13196276317384 23.13516037552859 18-19 22.51242317022224 26.113016449948063 29.043116288046043 22.331444091783656 20-21 23.317107207391548 25.543360685607453 28.95183250767549 22.187699599325505 22-23 23.108336277623806 25.282953712202854 28.774325905435898 22.83438410473744 24-25 22.751397153918106 25.413446767530207 28.8266907710784 23.00846530747329 26-27 22.635615003174124 25.362325145721147 28.970935678485652 23.031124172619073 28-29 22.64464217255403 25.84102958303968 28.94142362463219 22.5729046197741 30-31 23.49956883536898 25.44529189796691 28.85588270049244 22.199256566171673 32-33 23.09532205421802 25.605823648500127 28.767727710323964 22.53112658695789 34-35 23.198871427322164 25.7995691501569 28.629754562964404 22.371804859556534 36-37 23.20033067488998 25.781455573196443 28.49845297985589 22.519760772057687 38-39 23.17572067526925 26.01001245875711 28.278493436736696 22.53577342923694 40-41 23.144756131220408 25.72867847701118 28.515376112607445 22.611189279160964 42-43 22.92712587484479 25.91933166181315 28.13769143069805 23.015851032644004 44-45 22.803304116971507 26.050824892880048 28.23785281402499 22.908018176123456 46-47 22.82065644866106 25.78364367974988 28.018507227920942 23.377192643668117 48-49 22.820504123845712 25.993306453073608 28.01301063289125 23.17317879018943 50-51 22.774782693622424 26.32750002219677 27.911532553782774 22.986184730398033 52-53 22.864925481329028 26.458027941982426 27.4068049397912 23.270241636897346 54-55 22.624621540152365 26.195196284224508 27.830790885093354 23.34939129052977 56-57 22.859156786354895 26.318283122810303 27.38624010766658 23.436319983168218 58-59 22.80212211868463 26.33041033150092 27.71601813941468 23.151449410399774 60-61 23.237197640317014 26.369178602917962 27.67487494411679 22.71874881264823 62-63 23.00564473625925 26.368464826502947 27.66085882691005 22.965031610327756 64-65 22.761578266907605 26.143827344832744 27.58860955691049 23.50598483134916 66-67 22.77752413441502 26.232957525897444 27.42606276248426 23.563455577203275 68-69 22.505103491963844 26.273076678612988 27.543303781786083 23.67851604763709 70-71 22.620448207743383 26.094484768111275 27.615068184394588 23.66999883975076 72-73 22.52985178373873 26.530833275481903 27.163139090412113 23.776175850367252 74-75 22.64819801276664 26.632332531649155 26.971939337385876 23.74753011819833 76-77 22.43979990815171 26.263284644387934 27.30229957191026 23.994615875550092 78-79 22.405040355745953 26.360131610590955 27.264961081519967 23.96986695214313 80-81 22.507307031128285 26.64402985041503 26.88261301652443 23.96605010193225 82-83 22.61320067147637 26.575452762738212 26.97038455426806 23.840962011517362 84-85 22.64217137219355 26.766513360242516 26.621078060575048 23.970237206988884 86-87 22.337883437924663 26.728020640586834 26.89979472331323 24.03430119817527 88-89 22.13791545958948 26.44604695647882 27.046003600236983 24.370033983694718 90-91 22.745621133748813 26.36820151431168 26.952065143170028 23.934112208769488 92-93 22.530342551406413 26.51081586866547 26.853991343470106 24.104850236458002 94-95 22.240664069854883 26.314082251863518 26.89808527223514 24.54716840604646 96-97 22.08693350240527 26.35230992434463 27.090585197049915 24.470171376200184 98-99 22.21948212083847 26.32617208132597 26.80293196766207 24.65141383017349 100 22.06396759897266 26.40951109062651 26.920517561386646 24.60600374901419 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2695.0 1 2034.0 2 4218.5 3 5983.5 4 3501.0 5 2362.0 6 3246.0 7 5632.5 8 8566.5 9 9883.5 10 8539.5 11 6816.0 12 6734.0 13 7157.5 14 7762.0 15 8271.5 16 8741.5 17 9409.5 18 10067.5 19 10683.5 20 11430.5 21 12472.0 22 13861.0 23 15872.5 24 18379.0 25 21321.5 26 25735.5 27 31008.5 28 36245.0 29 42283.5 30 50130.0 31 57913.0 32 66250.5 33 76187.5 34 85786.5 35 94497.5 36 106478.0 37 118560.5 38 125343.0 39 132544.5 40 138185.0 41 143187.5 42 150786.0 43 159508.0 44 169129.5 45 179621.5 46 190458.5 47 202851.5 48 222061.0 49 235754.0 50 245144.5 51 244750.5 52 231355.0 53 224310.0 54 218260.5 55 205119.5 56 187783.0 57 172201.5 58 157487.0 59 138446.0 60 115172.0 61 93679.0 62 76887.0 63 60635.0 64 45561.5 65 34517.0 66 26092.5 67 20487.5 68 16042.5 69 12344.5 70 9685.0 71 7618.0 72 6224.5 73 5128.0 74 4033.5 75 2860.0 76 1999.5 77 1287.0 78 801.0 79 536.0 80 377.5 81 245.0 82 160.5 83 109.5 84 78.5 85 59.5 86 38.5 87 33.0 88 26.5 89 17.5 90 10.5 91 6.5 92 5.5 93 3.0 94 1.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04451015921747341 2 0.010173243411093841 3 0.0 4 3.018239755472868E-4 5 3.5508703005563146E-4 6 1.7754351502781576E-5 7 1.7754351502781576E-5 8 5.326305450834472E-5 9 0.0 10-11 8.877175751390788E-6 12-13 3.462098543042407E-4 14-15 0.012658852621483262 16-17 0.0016777862170128588 18-19 5.326305450834472E-5 20-21 7.9006864187378E-4 22-23 9.232262781446417E-4 24-25 0.004580622687717646 26-27 0.016813370873134153 28-29 0.013901657226677973 30-31 0.010341909750370267 32-33 0.007456827631168261 34-35 0.009400929120722844 36-37 0.005397322856845598 38-39 0.004243290009164796 40-41 0.005779041414155402 42-43 0.007510090685676605 44-45 0.007421318928162698 46-47 0.015845758716232554 48-49 0.018251473344859458 50-51 0.017257229660703692 52-53 0.011460433895045507 54-55 0.003897080154860555 56-57 0.003897080154860555 58-59 0.01060822502291199 60-61 0.0024856092103894205 62-63 1.1540328476808022E-4 64-65 2.840696240445052E-4 66-67 0.001074138265918285 68-69 7.10174060111263E-5 70-71 2.041750422819881E-4 72-73 0.0031780289189979015 74-75 0.005690269656641494 76-77 0.0054772174386081155 78-79 0.0035597474763077053 80-81 7.989458176251708E-5 82-83 0.0010119980356585497 84-85 3.6396420580702224E-4 86-87 4.2610443606675775E-4 88-89 2.663152725417236E-4 90-91 4.971218420778841E-4 92-93 4.704903148237117E-4 94-95 4.438587875695393E-5 96-97 0.0018730840835434561 98-99 4.7936749057510253E-4 100 1.420348120222526E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5632422.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.72118391686737 #Duplication Level Percentage of deduplicated Percentage of total 1 74.70922405970819 42.375956381771616 2 15.473983951971206 17.55405379332826 3 4.583741933530837 7.799858077177797 4 1.8489308883020714 4.194941958598351 5 0.9288837646338517 2.6343693425594426 6 0.5598959071540159 1.9054775234390542 7 0.3514623686436731 1.3954753156186934 8 0.2602514596578037 1.180941672630678 9 0.18820986518476288 0.9607937740302374 >10 0.9883271947293764 10.13587228335626 >50 0.06785414216325714 2.6563395907321063 >100 0.03587064735706846 3.637428382316626 >500 0.002176587844207497 0.8100232792415297 >1k 9.49783295584767E-4 1.017994520387841 >5k 1.1872291194808164E-4 0.4680651306283383 >10k+ 1.1872291194808164E-4 1.2724089741831652 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22999 0.4083323302124734 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20769 0.3687401263612705 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12917 0.22933295836142958 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9813 0.1742234512967956 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 5944 0.10553186533253367 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.7754351502781573E-5 0.0 5 0.0 0.0 0.0 1.7754351502781573E-5 0.0 6 1.7754351502781573E-5 0.0 0.0 1.7754351502781573E-5 0.0 7 1.7754351502781573E-5 0.0 0.0 1.7754351502781573E-5 0.0 8 1.7754351502781573E-5 0.0 0.0 1.7754351502781573E-5 0.0 9 1.7754351502781573E-5 0.0 0.0 1.7754351502781573E-5 0.0 10-11 2.663152725417236E-5 0.0 0.0 4.438587875695394E-5 2.6631527254172363E-5 12-13 3.5508703005563146E-5 0.0 0.0 5.3263054508344725E-5 9.764893326529866E-5 14-15 3.5508703005563146E-5 0.0 0.0 5.3263054508344725E-5 1.6866633927642498E-4 16-17 7.101740601112629E-5 0.0 0.0 5.3263054508344725E-5 2.130522180333789E-4 18-19 7.101740601112629E-5 0.0 0.0 5.3263054508344725E-5 2.130522180333789E-4 20-21 7.101740601112629E-5 0.0 0.0 7.989458176251708E-5 2.4856092103894205E-4 22-23 7.101740601112629E-5 0.0 0.0 2.751924482931144E-4 2.663152725417236E-4 24-25 7.101740601112629E-5 0.0 0.0 7.279284116140446E-4 2.8406962404450516E-4 26-27 7.101740601112629E-5 0.0 0.0 0.0016777862170128588 2.8406962404450516E-4 28-29 7.101740601112629E-5 0.0 0.0 0.004891323839016324 3.018239755472868E-4 30-31 7.101740601112629E-5 0.0 0.0 0.014807129153319835 3.018239755472868E-4 32-33 7.101740601112629E-5 0.0 0.0 0.04050655295359616 3.018239755472868E-4 34-35 1.0652610901668945E-4 0.0 0.0 0.07944184579919616 3.1070115129867756E-4 36-37 1.0652610901668945E-4 0.0 0.0 0.14057007802327312 3.1957832705006834E-4 38-39 1.0652610901668945E-4 0.0 0.0 0.2343485626609654 3.1957832705006834E-4 40-41 1.0652610901668945E-4 0.0 0.0 0.3516249315125891 3.1957832705006834E-4 42-43 1.0652610901668945E-4 0.0 0.0 0.45784033937797985 3.1957832705006834E-4 44-45 1.0652610901668945E-4 0.0 0.0 0.5840205155082485 3.284555028014591E-4 46-47 1.0652610901668945E-4 0.0 0.0 0.7332192083618735 3.373326785528499E-4 48-49 1.0652610901668945E-4 0.0 0.0 0.8924668641660728 3.373326785528499E-4 50-51 1.0652610901668945E-4 0.0 0.0 1.0589316638561528 3.373326785528499E-4 52-53 1.0652610901668945E-4 0.0 0.0 1.2290449827800545 4.1722726031536697E-4 54-55 1.0652610901668945E-4 0.0 0.0 1.421440012839947 4.971218420778841E-4 56-57 1.0652610901668945E-4 0.0 0.0 1.6389219415732699 4.971218420778841E-4 58-59 1.0652610901668945E-4 0.0 0.0 1.8795022816117117 4.971218420778841E-4 60-61 1.0652610901668945E-4 0.0 0.0 2.122310792763752 4.971218420778841E-4 62-63 1.0652610901668945E-4 0.0 0.0 2.381178114850059 5.326305450834472E-4 64-65 1.0652610901668945E-4 0.0 0.0 2.6441999551880166 5.326305450834472E-4 66-67 1.0652610901668945E-4 0.0 0.0 2.940262643672651 5.326305450834472E-4 68-69 1.2428046051947102E-4 0.0 0.0 3.2876709167033296 5.326305450834472E-4 70-71 1.331576362708618E-4 0.0 0.0 3.667543021456844 5.85893599591792E-4 72-73 1.5091198777364336E-4 0.0 0.0 4.016779992692309 6.391566541001367E-4 74-75 1.5978916352503417E-4 0.0 0.0 4.364685387565066 6.391566541001367E-4 76-77 1.5978916352503417E-4 0.0 0.0 4.740749183921233 6.391566541001367E-4 78-79 1.5978916352503417E-4 0.0 0.0 5.175455603291089 6.391566541001367E-4 80-81 1.5978916352503417E-4 0.0 0.0 5.61537292482701 6.835425328570906E-4 82-83 1.5978916352503417E-4 0.0 0.0 6.066058260549369 7.634371146196077E-4 84-85 1.5978916352503417E-4 0.0 0.0 6.520223449166274 7.723142903709985E-4 86-87 1.5978916352503417E-4 0.0 0.0 7.0025026533878325 7.9006864187378E-4 88 1.5978916352503417E-4 0.0 0.0 7.389680673784741 7.989458176251708E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 11815 0.0 59.107212 2 ACCTGGG 13320 0.0 51.472084 3 GTACCTG 13725 0.0 51.110313 1 TATAACG 785 0.0 40.709393 2 CCTGGGG 11110 0.0 40.13958 4 ATAACGC 890 0.0 34.84774 3 TAACGCA 895 0.0 34.653057 4 CTGGGGG 6480 0.0 29.224743 5 GTACCCG 1175 0.0 27.6098 1 GGTATCA 24475 0.0 24.319973 1 TGGGGGG 9740 0.0 24.219728 6 GTATAAC 1620 0.0 24.088797 1 GTATCAA 31785 0.0 22.661497 1 TATCACG 500 0.0 22.55779 2 ATGGGGG 8575 0.0 22.194305 5 TACCCGG 1430 0.0 21.690186 2 CATGGGG 14200 0.0 21.609539 4 GTACATG 27445 0.0 21.174213 1 TACATGG 27960 0.0 20.489052 2 GTACACG 1200 0.0 19.982094 1 >>END_MODULE