FastQCFastQC Report
Mon 6 Jun 2016
ERR522884_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522884_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8303854
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT690470.8315054672204015No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT263920.3178283240529036No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207030.24931796729566777No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT203140.24463339552935298No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC153700.18509477647367115No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC140220.16886135040428216No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118300.14246396914011253No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG112960.13603322023725367No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA99150.11940238833678916No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG302550.027.3978181
TACATGG311600.026.1919862
ACATGGG317300.025.0857263
CATGGGG221300.023.1768424
ATGGGGG129850.021.3255395
GAGTACT223300.020.48241412-13
GTACTTT243050.018.72132514-15
TACCTGG58450.018.579912
AGAGTAC371650.018.50524710-11
AGTACTT237600.017.97427612-13
GAGTACA240950.016.8596151
AGTACAT232050.016.2888582
TATAACG7050.016.0043582
GTACCTG74050.016.00061
TGGGGGG130550.015.6901266
ACTTTTT293650.015.59753816-17
GTATAGG25050.015.5786981
CATGGGA111400.015.2768684
GTATAGA23800.015.211591
GTATAAT40200.014.8538321
Produced by FastQC (version 0.11.5)