FastQCFastQC Report
Mon 6 Jun 2016
ERR522884_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522884_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8303854
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT702640.84616131256643No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT236380.28466300105950804No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT202340.24366998745401833No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197280.23757643137752665No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC160200.19292246708576524No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC145260.1749308212788905No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA116340.14010361935554264No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112300.1352384085751026No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA102570.12352095785884482No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG297500.027.7119451
TACATGG305650.026.4810312
ACATGGG307250.025.6355633
CATGGGG210200.023.6768534
ATGGGGG123350.021.0302775
GAGTACT224700.020.73287212-13
TACCTGG58050.020.2424892
GTACTTT240950.019.03898414-15
AGAGTAC368200.018.70429810-11
AGTACTT241750.018.08445412-13
GTACCTG71600.017.274871
CATGGGA109800.016.9493714
GAGTACA235550.016.4119721
TGGGGGG116950.015.91255956
ACTTTTT290100.015.86777916-17
AGTACAT225700.015.681572
TACCGTA4852.910383E-1115.5031757
TACTTTT282650.015.19910814-15
TAACGCA8350.015.1963064
ATAACGC9600.015.1757873
Produced by FastQC (version 0.11.5)