FastQCFastQC Report
Mon 6 Jun 2016
ERR522883_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522883_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9884121
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT383310.387803832025124No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT363760.3680246326405757No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290580.29398668834588326No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187490.18968808657846256No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134210.13578344498210818No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA107910.10917511026018399No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG164900.054.0961842
GTACCTG189600.047.778281
ACCTGGG191650.045.736113
CCTGGGG174650.033.653184
TATAACG9550.027.0819072
CATGGGG255250.022.9160274
CTGGGGG113350.022.7311635
ATAACGC12250.022.2645573
GTACATG482850.022.0826821
ATGGGGG150750.021.3646555
TACATGG498550.021.0054232
TGGGGGG157950.020.8565676
GTACACG24350.020.474791
ACATGGG498100.020.4391823
TAACGCA12650.019.7001724
GAGTACT308750.019.57275812-13
GTACCGG17950.019.390091
TATCACG10050.019.1839372
TACCGGG14450.018.5492762
AGTACTT326600.017.5965812-13
Produced by FastQC (version 0.11.5)