FastQCFastQC Report
Mon 6 Jun 2016
ERR522883_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522883_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9884121
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT384490.3889976660544726No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT316860.32057478859273375No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT282920.28623688439265366No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183560.1857120122264792No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118720.12011184403752241No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA101560.10275066442428216No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG170600.054.1333772
GTACCTG195850.047.559111
ACCTGGG196350.046.358543
CCTGGGG172450.034.716614
TATAACG9850.032.4454842
CTGGGGG109750.024.7486885
TAACGCA13450.024.1078174
ATAACGC14300.023.6606963
CATGGGG244350.021.5203234
TGGGGGG144250.021.174936
GTACATG483250.020.4810891
ATGGGGG143750.020.2353925
TACATGG499850.019.5665382
ACATGGG493300.019.176253
GTACACG24550.019.1523171
GTATAAC24950.018.6568131
GAGTACT321000.018.6107512-13
TATCACG9850.018.6084382
AGTACTT337150.017.59379412-13
GTACCCG17450.017.5142151
Produced by FastQC (version 0.11.5)