FastQCFastQC Report
Mon 6 Jun 2016
ERR522882_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522882_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4506774
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT328640.7292134018701626No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT93050.20646697615633708No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC61260.1359287153072242No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC45490.10093694514080359No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA45240.10038222462453188No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG48450.044.0587432
GTACCTG62300.035.175121
GTACATG177250.034.6227871
TACATGG184650.032.848112
ACCTGGG64400.032.3453
ATGGGGG95900.032.1618275
ACATGGG186900.031.7245273
CATGGGG143050.030.994124
TGGGGGG90650.028.6595556
TATAACG3450.023.1686022
CCTGGGG64850.022.9103954
ATAACGC4500.021.9430073
GTATATA11950.019.2826161
GTATTAT10900.017.6886671
ATAAGTA13900.017.2508665
TAACGCA5200.017.1793124
TAAGTAC34500.017.0217046
ATATCTA10250.016.9752271
GTACAGG34450.016.7901131
GTATAGA12800.016.5325661
Produced by FastQC (version 0.11.5)