FastQCFastQC Report
Mon 6 Jun 2016
ERR522882_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR522882_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4506774
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT338470.7510250125699669No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT94770.2102834533082866No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC66560.14768879025218482No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC64070.14216377391011842No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG51900.11515997917801071No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA48390.10737170312955566No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG51250.042.6471632
ACCTGGG63500.033.8217773
GTACCTG67050.033.318561
GTACATG179600.033.2836231
TACATGG188150.031.2524572
ACATGGG191500.030.0867773
CATGGGG145800.029.5251374
TGGGGGG85150.029.0850816
ATGGGGG98850.028.5245575
TATAACG3500.026.8591982
CCTGGGG63500.024.9407864
GTATATA10500.021.9481621
ATAAGTA13150.020.3701135
CTGGGGG37300.019.5284235
TAAGTAC36500.018.411436
ATAACGC4203.6379788E-1217.9029523
GAGTACA129850.017.85651
GTATATG13100.017.5920411
AGTACAT124550.017.2466032
ATATGTA17650.017.0540161
Produced by FastQC (version 0.11.5)